Douglas G R, Bell R D, Grant C E, Wytsma J M, Bora K C
Mutat Res. 1980 Feb;77(2):157-63. doi: 10.1016/0165-1218(80)90133-0.
Possible mutagenic activity of lead chromate in mammalian cells was studied using assays for chromosome aberrations and sister-chromatid exchanges in cultured human lymphocytes, and DNA fragmentation as detected by alkaline-sucrose gradient sedimentation in cultured Chinese hamster ovary (CHO) cells. Lead chromate caused dose-related increases in chromosome aberration and sister-chromatid exchange in human lymphocytes. No increase in DNA damage was observed in CHO cells, possibly due to the relative insensitivity of the CHO cells and the limited solubility of lead chromate in tissue culture medium. The mutagenicity of lead chromate in human lymphocytes appears to be entirely due to the chromate ion since chromosome aberrations were induced by potassium chromate but not lead chloride.
利用检测培养的人淋巴细胞中的染色体畸变和姐妹染色单体交换，以及通过碱性蔗糖梯度沉降检测培养的中国仓鼠卵巢（CHO）细胞中的DNA片段化，研究了铬酸铅在哺乳动物细胞中的潜在诱变活性。铬酸铅导致人淋巴细胞中染色体畸变和姐妹染色单体交换呈剂量相关增加。在CHO细胞中未观察到DNA损伤增加，这可能是由于CHO细胞相对不敏感以及铬酸铅在组织培养基中的溶解度有限。铬酸铅在人淋巴细胞中的致突变性似乎完全归因于铬酸根离子，因为铬酸钾可诱导染色体畸变，而氯化铅则不能。
