兔肝微粒体将葡萄糖转化为类固醇的机制。

Mechanism of glucose transfer to steroids by rabbit liver microsomes.

作者信息

Comerton A M, Layne D S, Williamson D G

出版信息

Can J Biochem. 1978 Nov;56(11):1082-5. doi: 10.1139/o78-171.

Abstract

The formation of the 3-glucosides of estrone, 17alpha-estradiol, or 17beta-estradiol by washed rabbit liver microsomes in the absence of UDP glucose (UDPG) was increased by the addition of crude lipid extracts of rabbit or pig liver and of dolichol or retinol. The reaction was stimulated by Mn2+, Mg2+, and Ca2+. Direct transfer of glucose to the steroid from added UDPG also occurred by a mechanism which was inhibited by metal ions. The two transferases can be separated from each other into the 105000 g pellet and supernatant respectively by treatment with detergents.

摘要

在没有尿苷二磷酸葡萄糖（UDPG）的情况下，经洗涤的兔肝微粒体形成雌酮、17α-雌二醇或17β-雌二醇的3-葡萄糖苷的过程，会因添加兔或猪肝的粗脂质提取物以及多萜醇或视黄醇而增强。该反应受到Mn2 +、Mg2 +和Ca2 +的刺激。从添加的UDPG将葡萄糖直接转移至甾体也会通过一种被金属离子抑制的机制发生。通过用去污剂处理，这两种转移酶可分别被分离到105000g沉淀和上清液中。