Determination of sodium saccharin in animal feed, wastewater and human urine by high-pressure liquid chromatography.

Analytical methods are described for sodium saccharin in animal feed, wastewater and human urine at levels as low as 10, 0.1 and 10 ppm, respectively. Samples of animal feed and wastewater are subjected to liquid-liquid partitioning then the feed is further cleaned up on a column of silica gel prior to analysis by high-pressure liquid chromatography (HPLC) using a paired-ion mobile phase and an ultraviolet detector set at 230 nm. Samples of human urine require a cleanpu on a column of XAD-2 prior to the partitioning and silica gel steps as well as an adjustment in the composition of the mobile phase to quantify saccharin. Data concerning partition values and the stability of sodium saccharin in animal feed are also presented.