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建立一种直接竞争酶联免疫吸附分析法用于食品中糖精钠残留量的定量检测。

Development of a direct competitive enzyme-linked immunosorbent assay for quantitation of sodium saccharin residue in food.

机构信息

College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.

Department of Entomology and UCD Comprehensive Cancer Center, University of California, Davis, California, USA.

出版信息

J Food Sci. 2021 Aug;86(8):3720-3729. doi: 10.1111/1750-3841.15767. Epub 2021 Jul 26.

Abstract

Sodium saccharin is a common artificial sweetener. However, due to its possible carcinogenic effects and causing metabolic disorders, many countries have strictly regulated its use in food. In the study, we prepared a specific monoclonal antibody (mAb 2H11) using the new hapten (6-carboxylsaccharin) and developed a direct competitive enzyme-linked immunosorbent assay (dcELISA) for the screening of sodium saccharin residue in food. The half-maximum inhibition concentration (IC ) and working range (IC -IC , the concentrations causing 20% and 80% inhibition by sodium saccharin) were 32.5 and 6.47 to 164 ng/mL, which was 6.5 times more sensitive than the previously reported immunoassay. The average recoveries of sodium saccharin in spiked food samples detected by dcELISA ranged from 82.1% to 117%. Among 70 food samples bought in the physical stores and online, sodium saccharin residues were only detected in four samples purchased online (one canned pineapple, two winter jujube, and one kimchi). The content measured by dcELISA agreed well with those determined by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The developed dcELISA was proved to be a sensitive and accurate method for determining sodium saccharin in food. PRACTICAL APPLICATION: Quantitation of sodium saccharin residue in food is very necessary and important for consumers and regulatory agencies.

摘要

糖精钠是一种常见的人工甜味剂。然而,由于其可能的致癌作用和引起代谢紊乱,许多国家已经严格限制了其在食品中的使用。在这项研究中,我们使用新的半抗原(6-羧基糖精)制备了一种特异性单克隆抗体(mAb 2H11),并开发了一种用于食品中糖精钠残留筛选的直接竞争酶联免疫吸附测定法(dcELISA)。半最大抑制浓度(IC )和工作范围(IC -IC ,糖精钠引起 20%和 80%抑制的浓度)分别为 32.5 和 6.47 至 164ng/mL,比之前报道的免疫分析方法灵敏 6.5 倍。dcELISA 检测添加到食品样品中的糖精钠的平均回收率在 82.1%至 117%之间。在实体店和网上购买的 70 个食品样品中,仅在 4 个网上购买的样品中检测到糖精钠残留(一个罐装菠萝、两个冬枣和一个泡菜)。dcELISA 测定的含量与超高效液相色谱-串联质谱法(UPLC-MS/MS)测定的含量吻合良好。开发的 dcELISA 被证明是一种用于测定食品中糖精钠的灵敏和准确的方法。

实际应用

对食品中糖精钠残留量的定量检测对消费者和监管机构来说是非常必要和重要的。

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