Jubb R W, Fell H B
Arthritis Rheum. 1980 May;23(5):545-55. doi: 10.1002/art.1780230505.
Explants of pig articular cartilage were grown in organ culture in the presence of synovial tissue; controls consisted of paired explants that were cultivated in isolation. To find whether the synovial tissue affected synthesis of sulfated proteoglycan by the cartilage, 35SO4 was added to the medium and its incorporation into the cartilage examined by both biochemical assay and autoradiography. The synovial tissue severely inhibited the uptake of 35SO4, but if the synovium was removed after 8 days cultivation and the cartilage was grown in isolation for a further 4 days, incorporation of 35SO4 equalled and sometimes surpassed that of controls which had been grown without synovium continuously for 12 days. Synovium did not prevent the formation of new cartilage on the cut surfaces of the explants, but it reduced the incidence of newly formed cartilage.
猪关节软骨外植体在滑膜组织存在的情况下进行器官培养；对照组由单独培养的配对外植体组成。为了确定滑膜组织是否影响软骨硫酸化蛋白聚糖的合成，向培养基中添加了35SO4，并通过生化分析和放射自显影检查其在软骨中的掺入情况。滑膜组织严重抑制了35SO4的摄取，但如果在培养8天后去除滑膜，软骨再单独培养4天，35SO4的掺入量与连续12天无滑膜培养的对照组相当，有时甚至超过对照组。滑膜并不阻止外植体切割面上新软骨的形成，但它降低了新形成软骨的发生率。
