Steinberg J J, Kincaid S B, Sledge C B
Ann Rheum Dis. 1983 Jun;42(3):323-30. doi: 10.1136/ard.42.3.323.
Bovine nasal cartilage discs cocultured with human rheumatoid synovial membrane or synovial-membrane-conditioned media release proteoglycan largely as a result of cartilage breakdown. We assessed the effects of hydrocortisone on proteoglycan distribution between cartilage and culture medium, and on cartilage breakdown expressed as the release of either proteoglycan or 35S-products from prelabelled discs. The presence of synovial membrane inhibited the capacity for net proteoglycan synthesis, preventing its accumulation in cartilage; this was little affected by hydrocortisone. The major response to pharmacological concentrations of hydrocortisone was suppression of both spontaneous and synovial-membrane-induced cartilage breakdown. The autolysis of synovial protein that normally occurred during culture was similarly prevented by comparable doses of corticosteroid. Changes in chromatographic distribution of the 35S-labelled degradation products released from cartilage conformed with a corticosteroid-induced inhibition of endogenous lysosomal or related proteinase activity. Additionally, inhibition of the early events in synovial membrane that are responsible for chondrocyte-mediated breakdown of cartilage may contribute significantly to the overall corticosteroid effect.
与人类类风湿性滑膜或滑膜条件培养基共培养的牛鼻软骨盘释放蛋白聚糖，这主要是软骨分解的结果。我们评估了氢化可的松对软骨与培养基之间蛋白聚糖分布的影响，以及对软骨分解的影响，软骨分解表现为蛋白聚糖或来自预先标记盘的35S产物的释放。滑膜的存在抑制了蛋白聚糖净合成的能力，阻止其在软骨中积累；氢化可的松对此影响不大。对药理浓度氢化可的松的主要反应是抑制自发性和滑膜诱导的软骨分解。在培养过程中通常发生的滑膜蛋白自溶，也被相当剂量的皮质类固醇同样阻止。从软骨释放的35S标记降解产物的色谱分布变化符合皮质类固醇诱导的内源性溶酶体或相关蛋白酶活性的抑制。此外，抑制滑膜中负责软骨细胞介导的软骨分解的早期事件，可能对皮质类固醇的总体作用有显著贡献。
