The effects of pH on the reductions kinetics of P-680 in Tris-treated chloroplasts.

The primary donor of Photosystem II (PS II), P-680, was photo-oxidized by a short flash and its rate of reduction was measured at different pH values by following the recovery of the absorption change at 820 nm in chloroplasts pretreated with a high concentration of Tris. The re-duction is biphasic with a fast phase (dominant after the first flash) attributed to the donation by a donor, D1, and a slow phase (usually dominant after the second flash) attributed to a back-reaction with the primary acceptor. It is found that pH has a strong influence on the donation from D1 (PI = 2 MICROSECONDS AT PH 9, 44 microseconds at pH 4), but no influence on the back reaction (pi approximately 200 microseconds). pH also influences the stability of the charge separation since the contribution of donation from D1 at the second flash increases at lower pH, getting close to 100% at pH 4.