Dutta P K, Spencer R, Walsh C, Spiro T G
Biochim Biophys Acta. 1980 May 29;623(1):77-83. doi: 10.1016/0005-2795(80)90009-4.
Resonance Raman and coherent anti-stokes Raman scattering spectra are reported for flavins modified by deprotonation at N3 and by CH substitution for N1, N3 and N5, using laser excitation in resonance with the visible electronic transition. Vibrational shifts are used to make qualitative assignments of the observed vibrational modes. Both 8-CH3NH- and ionized 8-OH-riboflavin were found to give markedly different spectra patterns form flavin itself; this observation supports a partial zwitterionic structure for the 8-CH3NH derivatives.
报道了通过N3去质子化以及N1、N3和N5的CH取代修饰的黄素的共振拉曼光谱和相干反斯托克斯拉曼散射光谱，使用与可见电子跃迁共振的激光激发。振动位移用于对观察到的振动模式进行定性归属。发现8-CH3NH-和离子化的8-OH-核黄素都给出了与黄素本身明显不同的光谱模式；这一观察结果支持了8-CH3NH衍生物的部分两性离子结构。
