Hasany S M, Basu R, Basu P K, Lin B J
Can J Ophthalmol. 1980 Apr;15(2):76-80.
We wished to measure the amount of reduced nitroblue tetrazolium (NBT) formed in corneal cells and to relate it to the viability of the cells in terms of their oxygen uptake. Two full-thickness corneal discs of identical size (3, 4, 5 or 6 mm in diameter) were cut from the eyes of guinea pigs. One disc was processed for the measurement of its reduced NBT and cellular nitrogen content; the other was processed for the measurement of its oxygen uptake and deoxyribonucleic acid (DNA) content. As the diameter of the corneal disc (that is, the number of cells) increased, the quantity of reduced NBT, nitrogen and DNA and the oxygen uptake increased proportionality. Since each of these measures correlated positively with each other (P less than 0.01) we were able to estimate the number of viable cells in a given cornea by measuring the amount of reduced NBT (formazan granules) formed in the cells.
我们希望测量角膜细胞中形成的还原型硝基蓝四氮唑（NBT）的量，并根据细胞的氧摄取情况将其与细胞活力相关联。从豚鼠眼中切下两个大小相同（直径3、4、5或6毫米）的全层角膜片。一个角膜片用于测量其还原型NBT和细胞氮含量；另一个角膜片用于测量其氧摄取和脱氧核糖核酸（DNA）含量。随着角膜片直径（即细胞数量）的增加，还原型NBT、氮和DNA的量以及氧摄取量成比例增加。由于这些测量指标彼此之间呈正相关（P小于0.01），我们能够通过测量细胞中形成的还原型NBT（甲臜颗粒）的量来估计给定角膜中活细胞的数量。
