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鼠伤寒沙门氏菌中第二种L-脯氨酸转运系统的生物化学与调控

Biochemistry and regulation of a second L-proline transport system in Salmonella typhimurium.

作者信息

Anderson R R, Menzel R, Wood J M

出版信息

J Bacteriol. 1980 Mar;141(3):1071-6. doi: 10.1128/jb.141.3.1071-1076.1980.

Abstract

This paper reports some biochemical characteristics of a second L-proline transport system in Salmonella typhimurium. In the accompanying paper, R. Menzel and J. Roth (J. Bacteriol. 141:1064--1070, 1980) have identified this system by showing that it is inactivated by mutations at the locus proP. We have found that it is an active transport system with an apparent Km for L-proline of 3 x 10(-4) M and a strict specificity for L-proline and some of its analogs. Unlike the L-proline transport system encoded in putP, this second system is induced by amino acid limitation.

摘要

本文报道了鼠伤寒沙门氏菌中第二个L-脯氨酸转运系统的一些生化特性。在随附的论文中,R. 门泽尔和J. 罗斯(《细菌学杂志》141:1064 - 1070,1980年)通过表明该系统因proP位点的突变而失活,从而鉴定出了这个系统。我们发现它是一个主动转运系统,对L-脯氨酸的表观Km值为3×10⁻⁴ M,对L-脯氨酸及其一些类似物具有严格的特异性。与putP中编码的L-脯氨酸转运系统不同,这个第二个系统是由氨基酸限制诱导的。

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