Karwacka H
Exp Pathol (Jena). 1980;18(2):118-26. doi: 10.1016/s0014-4908(80)80061-5.
The aim in view was to establish whether chronic administration of ethanol causes ultrastructura changes in the brain of rats, particularly in the structures through which alcohol penetrates to the brain, that is the cerebral cortex capillaries. The experiments were performed with 5 Wistar rats, three of which received ethanol according to RATCLIFFE'S model for 8 weeks in increasing concentrations from 2.5 to 25 per cent. Two rats served as control. In the endothelium of some capillaries of the brain cortex in the rats ingesting ethanol an enlargement of the cell nuclei was observed. The number of mitochondria in the cytoplasm and of micropinocytic vesicles was found to increase, and proliferation of the smooth endoplasmic reticulum and Golgi system were noted. Considerable oedema was observed in the astrocytic processes surrounding the vessels, with the presence of numerous mitochondria of abnormal shape and huge size. Oedema of perivascular astrocytic processes and enhanced pinocytosis seem to indicate an increased permeability of the blood-brain barrier as the result of the toxic effect of ethanol.
本研究的目的是确定长期给予乙醇是否会导致大鼠大脑出现超微结构变化，特别是在酒精进入大脑的结构中，即大脑皮质毛细血管。实验选用了5只Wistar大鼠，其中3只按照拉特克利夫模型，以浓度从2.5%至25%递增的方式给予乙醇，持续8周。2只大鼠作为对照。在摄入乙醇的大鼠大脑皮质的一些毛细血管内皮中，观察到细胞核增大。发现细胞质中的线粒体数量和微胞饮小泡数量增加，同时注意到滑面内质网和高尔基体系统增生。在血管周围的星形胶质细胞突起中观察到相当程度的水肿，存在许多形状异常且体积巨大的线粒体。血管周围星形胶质细胞突起的水肿和增强的胞饮作用似乎表明，由于乙醇的毒性作用，血脑屏障的通透性增加。
