Martin R J, Herbein J H, Sherritt G W, Wangsness P J
Growth. 1980 Mar;44(1):1-11.
In vitro glucose and pyruvate utilization by perinatal pig liver was determined at 90, 100, 110, and 114 days fetal age, and at 1 and 24 hours postpartum. The in vitro response of newborn pig liver to elevated glucose and insulin in vivo was also determined. Temporal patterns of increasing CO2 and total lipid and decreasing fatty acid production from glucose were associated with a decrease and subsequent increase in total liver DNA and protein at 110 days. A low capacity for gluconeogenesis from pyruvate was evident at 90 days fetal age, but doubled by 100 days then increased four-fold between 1 and 24 hours postpartum. In newborn pigs, the in vitro rate of glucose incorporation into CO2 and total lipids was increased by glucose feeding one hour before sacrifice, whereas fatty acid synthesis was increased by glucose feeding and/or intraperitoneal insulin injection. The in vivo glucose and/or insulin treatments also decreased serum growth hormone and cortisol levels. The results indicate that developmental changes in glucose utilization and synthesis by perinatal pig liver might be associated with changes in liver DNA content, whereas short-term changes at birth are related to serum concentrations of glucose and insulin.
在胎儿期90、100、110和114天以及出生后1小时和24小时，测定了围产期猪肝对体外葡萄糖和丙酮酸的利用情况。还测定了新生猪肝在体内对升高的葡萄糖和胰岛素的体外反应。在110天时，葡萄糖产生二氧化碳和总脂质增加以及脂肪酸产生减少的时间模式与肝脏总DNA和蛋白质先减少后增加有关。在胎儿期90天时，丙酮酸糖异生能力较低，但到100天时翻倍，然后在出生后1至24小时之间增加了四倍。在新生猪中，在处死前一小时喂食葡萄糖可提高体外葡萄糖掺入二氧化碳和总脂质的速率，而喂食葡萄糖和/或腹腔注射胰岛素可增加脂肪酸合成。体内葡萄糖和/或胰岛素处理也降低了血清生长激素和皮质醇水平。结果表明，围产期猪肝葡萄糖利用和合成的发育变化可能与肝脏DNA含量的变化有关，而出生时的短期变化与血清葡萄糖和胰岛素浓度有关。
