Ishikawa S, Iizuka A, Yanaura S
Nihon Yakurigaku Zasshi. 1980 Jan;76(1):25-32.
Mechanisms of hepatic bile secretion were studied in unanesthetized bile fistula rats. After implantation of bile fistula, bile flow and bile acids secretion were significantly decreased and secondary bile acids were greatly decreased. After cholic acid (CA) 30 microM/100 g/hr infusion, bile juice was greatly increased, and CA was dramatically increased in bile. Biliary bile flow and bile acid were not increased after infusion of CDCA 30 microM/100 g/hr. CDCA was slightly increased in bile, but the amount of bile acids tended to decrease. DHCA dramatically increased the amount of bile juice, while DHCA was not detected in the bile. After DHCA infusion, 3 alpha, 7 alpha-dihydroxy-12-keto-5 beta-cholanic acid dramatically increased and slightly increased in CA. Regarding the relationship between the bile flow rate and the secretion rate of the total bile acids, bile acid-dependent bile secretion varied with the bile acid value. On the other hand, Na+ and K+ concentrations of bile were not related to bile acid concentration. These studies show that canalicular bile secretion mechanisms varied with the compositions of bile acids and bile acid secretion rate.
在未麻醉的胆瘘大鼠中研究了肝脏胆汁分泌的机制。植入胆瘘后，胆汁流量和胆汁酸分泌显著减少，次级胆汁酸大幅减少。以30微摩尔/100克/小时的速度输注胆酸（CA）后，胆汁量大幅增加，胆汁中的CA显著增加。以30微摩尔/100克/小时的速度输注鹅去氧胆酸（CDCA）后，胆汁流量和胆汁酸并未增加。胆汁中的CDCA略有增加，但胆汁酸的量趋于减少。二氢胆酸（DHCA）显著增加了胆汁量，而胆汁中未检测到DHCA。输注DHCA后，3α，7α-二羟基-12-酮-5β-胆烷酸显著增加，CA略有增加。关于胆汁流速与总胆汁酸分泌率之间的关系，胆汁酸依赖性胆汁分泌随胆汁酸值而变化。另一方面，胆汁中的Na+和K+浓度与胆汁酸浓度无关。这些研究表明，胆小管胆汁分泌机制随胆汁酸组成和胆汁酸分泌率而变化。
