Itaya T, Tomioka H, Kumagai A, Tada T
Int Arch Allergy Appl Immunol. 1980;62(4):389-96. doi: 10.1159/000232541.
Effects of administration of various adjuvants were examined for the induction of IgE antibody response against a hapten-carrier conjugate in the mouse. It was confirmed that alum was an excellent adjuvant for induction of IgE antibody response, while poly A:U, CFA and LPS had much less activity. However, when these adjuvants were administered before immunization with antigen and alum, they suppressed the forthcoming antibody response to various degrees. Even alum exerted a profound suppressive effect when it was given before immunization. Such suppressive effect of alum and poly A:U was partially overcome by incorporating in the adjuvant the carrier protein. In contrast the suppressive effect of CFA was overcome by incorporating in the CFA an unrelated carrier conjugated to the same hapten (DNP). The results indicate that the target of the adjuvants is different from one to the other and further give a clue to study the possible application for the suppression of IgE antibody formation.
研究了各种佐剂对小鼠中诱导针对半抗原-载体结合物的IgE抗体应答的作用。已证实明矾是诱导IgE抗体应答的优良佐剂，而聚A:U、弗氏完全佐剂（CFA）和脂多糖（LPS）的活性则低得多。然而，当在抗原和明矾免疫前给予这些佐剂时，它们会不同程度地抑制随后的抗体应答。即使在免疫前给予明矾，也会产生显著的抑制作用。通过在佐剂中加入载体蛋白，明矾和聚A:U的这种抑制作用得到部分克服。相比之下，通过在CFA中加入与相同半抗原（二硝基苯，DNP）偶联的无关载体，CFA的抑制作用得以克服。结果表明，不同佐剂的作用靶点各不相同，这进一步为研究抑制IgE抗体形成的可能应用提供了线索。
