Mahieu P R, Foidart J B, Dubois C H, Dechenne C A, Deheneffe J
Invest Cell Pathol. 1980 Apr-Jun;3(2):121-8.
Rat glomerular epithelial and mesangial cells, and rat skin fibroblasts, were cultured in RPMI medium containing 15% decomplemented fetal bovine serum. They were incubated for 1 h at 37 degrees C with 2 mM [Ca2+]. At that time, their response to various concentrations of angiotensin II, norepinephrine and carbamylcholine was studied by phase contrast microscopy. Only the mesangial cells exhibited contractile activity in the presence of 10(-10) M angiotensin II and 10(-6) M norepinephrine, as demonstrated by the reduction of their length or, more frequently, by modification of their shape. No contractile activity was observed in the presence of 10(-5) M carbamylcholine. The percentage of contractile cells increased proportionally to the concentration of angiotensin II, reaching about 35% with 10(-6) M angiotensin II. The contractile activity was reversible. Specific inhibitors, i.e. Sar1-ala8-angiotensin II and phenoxybenzamine, when administered in concentrations 10 to 100-fold higher than that of the corresponding agonists, completely blocked the contractile response. This competitive effect was also reversible. The data, therefore, suggest that mesangial cells in culture display some functional properties of smooth muscle cells.
将大鼠肾小球上皮细胞、系膜细胞和大鼠皮肤成纤维细胞培养于含15% 灭能胎牛血清的RPMI培养基中。在37℃下用2 mM [Ca2+]孵育1小时。此时，通过相差显微镜研究它们对不同浓度血管紧张素II、去甲肾上腺素和卡巴胆碱的反应。仅系膜细胞在存在10(-10) M血管紧张素II和10(-6) M去甲肾上腺素时表现出收缩活性，表现为细胞长度缩短，更常见的是细胞形态改变。在存在10(-5) M卡巴胆碱时未观察到收缩活性。收缩细胞的百分比与血管紧张素II的浓度成比例增加，在10(-6) M血管紧张素II时达到约35%。收缩活性是可逆的。特异性抑制剂，即Sar1-ala8-血管紧张素II和酚苄明，当以比相应激动剂高10至100倍的浓度给药时，完全阻断收缩反应。这种竞争性作用也是可逆的。因此，数据表明培养的系膜细胞表现出平滑肌细胞的一些功能特性。
