Kurtz A, Jelkmann W, Sinowatz F, Bauer C
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4008-11. doi: 10.1073/pnas.80.13.4008.
Mesangial cells derived from isolated glomeruli of rat kidney were grown as homogeneous cell lines in culture. They released, into the culture medium, erythropoietin that had free terminal galactosyl residues and was therefore not active in vivo. The production of erythropoietin by these cells was significantly enhanced by either lowering the PO2 in the incubation atmosphere or by adding cobalt chloride to the culture medium. Therefore, mesangial cells in culture may be considered as an in vitro system in which the regulation of erythropoietin production can be studied under well-defined conditions.
从大鼠肾脏分离出的肾小球系膜细胞在培养中作为同质细胞系生长。它们向培养基中释放具有游离末端半乳糖基残基的促红细胞生成素，因此在体内无活性。通过降低孵育环境中的氧分压或向培养基中添加氯化钴，这些细胞促红细胞生成素的产生显著增强。因此，培养中的系膜细胞可被视为一种体外系统，在该系统中可以在明确界定的条件下研究促红细胞生成素产生的调节。
