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培养物中[精氨酸]加压素对磷脂酶C的激活及前列腺素的合成

Activation of phospholipase C and prostaglandin synthesis by [arginine]vasopressin in cultures.

作者信息

Pfeilschifter J, Kurtz A, Bauer C

出版信息

Biochem J. 1984 Nov 1;223(3):855-9. doi: 10.1042/bj2230855.

DOI:10.1042/bj2230855
PMID:6439189
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1144372/
Abstract

[Arginine]vasopressin (AVP) stimulates maximal prostaglandin E2 production in cultured rat renal mesangial cells within 2 min. As early as 10s after addition of AVP (10(-6)M) a significant loss of radioactivity from phosphatidylinositol 4,5-bisphosphate but not from phosphatidylinositol 4-phosphate and phosphatidylinositol was observed in cells prelabelled with 32Pi. Cells labelled with [14C]arachidonic acid showed an increase of label in 1,2-diacylglycerol after 15 s and in phosphatidic acid after 30 s upon stimulation with AVP. Pretreatment of the cells with indomethacin (10(-5)M) did not abolish the effect of AVP on the increased labelling of phosphatidic acid.

摘要

[精氨酸]血管加压素（AVP）在2分钟内刺激培养的大鼠肾系膜细胞中前列腺素E2的最大产量。早在加入AVP（10⁻⁶M）10秒后，在用³²Pi预标记的细胞中就观察到磷脂酰肌醇4,5 - 二磷酸有明显的放射性损失，但磷脂酰肌醇4 - 磷酸和磷脂酰肌醇没有。用[¹⁴C]花生四烯酸标记的细胞在用AVP刺激后，15秒时1,2 - 二酰基甘油中的标记增加，30秒时磷脂酸中的标记增加。用吲哚美辛（10⁻⁵M）预处理细胞并没有消除AVP对磷脂酸标记增加的影响。

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1
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2
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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
3
Direct demonstration of the formation of prostaglandin E2 due to phosphatidylinositol breakdown associated with stimulation of enzyme secretion in the pancreas.直接证明由于与胰腺中酶分泌刺激相关的磷脂酰肌醇分解而形成前列腺素E2。
J Biol Chem. 1981 Jan 25;256(2):844-7.
4
Tissue culture of normal rat glomeruli: contractile activity of the cultured mesangial cells.正常大鼠肾小球的组织培养：培养的系膜细胞的收缩活性。
Invest Cell Pathol. 1980 Apr-Jun;3(2):121-8.
5
Phospholipase A2 activity specific for phosphatidic acid. A possible mechanism for the production of arachidonic acid in platelets.对磷脂酸具有特异性的磷脂酶A2活性。血小板中花生四烯酸产生的一种可能机制。
J Biol Chem. 1981 Jun 10;256(11):5399-403.
6
Phospholipid and neutral lipid separation by one-dimensional thin-layer chromatography.通过一维薄层色谱法分离磷脂和中性脂质。
J Chromatogr. 1982 Oct 8;232(1):47-53. doi: 10.1016/s0378-4347(00)86006-5.
7
Mesangial cells derived from rat glomeruli produce an erythropoiesis stimulating factor in cell culture.源自大鼠肾小球的系膜细胞在细胞培养中产生促红细胞生成因子。
FEBS Lett. 1982 Jan 11;137(1):129-32. doi: 10.1016/0014-5793(82)80330-x.
8
Phosphatidylinositol metabolism in rat hepatocytes stimulated by vasopressin.血管加压素刺激下大鼠肝细胞中的磷脂酰肌醇代谢
Biochem J. 1981 Jan 15;194(1):155-65. doi: 10.1042/bj1940155.
9
How is the level of free arachidonic acid controlled in mammalian cells?哺乳动物细胞中游离花生四烯酸的水平是如何控制的？
Biochem J. 1982 Apr 15;204(1):3-16. doi: 10.1042/bj2040003.
10
Proposed mechanism of cholinergic action in smooth muscle.平滑肌中胆碱能作用的拟议机制。
Nature. 1980 Mar 27;284(5754):344-5. doi: 10.1038/284344a0.