Pfeilschifter J, Kurtz A, Bauer C
Biochem J. 1984 Nov 1;223(3):855-9. doi: 10.1042/bj2230855.
[Arginine]vasopressin (AVP) stimulates maximal prostaglandin E2 production in cultured rat renal mesangial cells within 2 min. As early as 10s after addition of AVP (10(-6)M) a significant loss of radioactivity from phosphatidylinositol 4,5-bisphosphate but not from phosphatidylinositol 4-phosphate and phosphatidylinositol was observed in cells prelabelled with 32Pi. Cells labelled with [14C]arachidonic acid showed an increase of label in 1,2-diacylglycerol after 15 s and in phosphatidic acid after 30 s upon stimulation with AVP. Pretreatment of the cells with indomethacin (10(-5)M) did not abolish the effect of AVP on the increased labelling of phosphatidic acid.
[精氨酸]血管加压素(AVP)在2分钟内刺激培养的大鼠肾系膜细胞中前列腺素E2的最大产量。早在加入AVP(10⁻⁶M)10秒后,在用³²Pi预标记的细胞中就观察到磷脂酰肌醇4,5 - 二磷酸有明显的放射性损失,但磷脂酰肌醇4 - 磷酸和磷脂酰肌醇没有。用[¹⁴C]花生四烯酸标记的细胞在用AVP刺激后,15秒时1,2 - 二酰基甘油中的标记增加,30秒时磷脂酸中的标记增加。用吲哚美辛(10⁻⁵M)预处理细胞并没有消除AVP对磷脂酸标记增加的影响。