培养物中[精氨酸]加压素对磷脂酶C的激活及前列腺素的合成
Activation of phospholipase C and prostaglandin synthesis by [arginine]vasopressin in cultures.
作者信息
Pfeilschifter J, Kurtz A, Bauer C
出版信息
Biochem J. 1984 Nov 1;223(3):855-9. doi: 10.1042/bj2230855.
Abstract
[Arginine]vasopressin (AVP) stimulates maximal prostaglandin E2 production in cultured rat renal mesangial cells within 2 min. As early as 10s after addition of AVP (10(-6)M) a significant loss of radioactivity from phosphatidylinositol 4,5-bisphosphate but not from phosphatidylinositol 4-phosphate and phosphatidylinositol was observed in cells prelabelled with 32Pi. Cells labelled with [14C]arachidonic acid showed an increase of label in 1,2-diacylglycerol after 15 s and in phosphatidic acid after 30 s upon stimulation with AVP. Pretreatment of the cells with indomethacin (10(-5)M) did not abolish the effect of AVP on the increased labelling of phosphatidic acid.
摘要
[精氨酸]血管加压素（AVP）在2分钟内刺激培养的大鼠肾系膜细胞中前列腺素E2的最大产量。早在加入AVP（10⁻⁶M）10秒后，在用³²Pi预标记的细胞中就观察到磷脂酰肌醇4,5 - 二磷酸有明显的放射性损失，但磷脂酰肌醇4 - 磷酸和磷脂酰肌醇没有。用[¹⁴C]花生四烯酸标记的细胞在用AVP刺激后，15秒时1,2 - 二酰基甘油中的标记增加，30秒时磷脂酸中的标记增加。用吲哚美辛（10⁻⁵M）预处理细胞并没有消除AVP对磷脂酸标记增加的影响。
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