聚氧乙烯二醇键合琼脂糖 6B 上的温和疏水相互作用层析一步法从短小芽孢杆菌中纯化脂肪酶。
One-Step Purification of a Lipolytic Enzyme from Bacillus pumilus by Mild Hydrophobic Interaction Chromatography on Polyoxyalkylene Glycol-Bound Sepharose 6B.
机构信息
Laboratoire de Chimie-Physique Macromoléculaire, Ecole Nationale Supérieure des Industries Chimiques, 1, Rue Grandville, 54042 Nancy Cedex, and Laboratoire de Microbiologie, Ecole Supérieure des Sciences et Technologies des Industries du Bois, Université de Nancy I, 54506 Vandoeuvre-lés-Nancy Cedex, France.
出版信息
Appl Environ Microbiol. 1988 May;54(5):1307-8. doi: 10.1128/aem.54.5.1307-1308.1988.
Abstract
An esterase from Bacillus pumilus was obtained in a highly purified form, starting from a crude extract, by a new chromatographic technique requiring no detergent during the elution step. The stationary phase involved was mildly hydrophobic and was prepared by covalent immobilization of a polyoxyalkylene glycol onto Sepharose 6B.
摘要
从粗提物中，通过一种新的色谱技术，在洗脱步骤中无需使用洗涤剂，即可获得高度纯化的解脂酶。所用的固定相具有中等疏水性，是通过将聚氧化烯二醇共价固定在 Sepharose 6B 上制备的。
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本文引用的文献
1
Surface affinity chromatographic separation of blood cells. I. Separation of human and rabbit peripheral granulocytes, lymphocytes and erythrocytes using polyethylene glycol-bonded column packings.血细胞的表面亲和色谱分离。I. 使用聚乙二醇键合柱填料分离人和兔外周血粒细胞、淋巴细胞及红细胞。
J Chromatogr. 1980 Jan 18;187(2):351-62. doi: 10.1016/s0021-9673(00)80467-7.
2
Enzyme kinetics of lipolysis.脂解作用的酶动力学
Methods Enzymol. 1980;64:340-92. doi: 10.1016/s0076-6879(80)64016-6.
3
Partial purification and characterization of lipase (EC 3.1.1.3) from Propionibacterium acnes.痤疮丙酸杆菌脂肪酶（EC 3.1.1.3）的部分纯化及特性研究
J Gen Microbiol. 1981 Jun;124(2):393-401. doi: 10.1099/00221287-124-2-393.
4
Purification and properties of carboxylesterase B of Escherichia coli.大肠杆菌羧酸酯酶B的纯化及性质
Ann Microbiol (Paris). 1984 May-Jun;135A(3):375-87. doi: 10.1016/s0769-2609(84)80079-4.
5
Maturation of the head of bacteriophage T4. I. DNA packaging events.噬菌体T4头部的成熟。I. DNA包装事件。
J Mol Biol. 1973 Nov 15;80(4):575-99. doi: 10.1016/0022-2836(73)90198-8.
6
A rapid, sensitive, and specific method for the determination of protein in dilute solution.一种用于测定稀溶液中蛋白质的快速、灵敏且特异的方法。
Anal Biochem. 1973 Dec;56(2):502-14. doi: 10.1016/0003-2697(73)90217-0.
7
Purification of microbial lipases by glass beads coated with hydrophobic materials.通过涂覆疏水材料的玻璃珠纯化微生物脂肪酶。
Chem Pharm Bull (Tokyo). 1977 Aug;25(8):1987-91. doi: 10.1248/cpb.25.1987.
8
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Annu Rev Biophys Bioeng. 1976;5:77-117. doi: 10.1146/annurev.bb.05.060176.000453.
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Biochim Biophys Acta. 1977 Sep 28;488(3):353-8. doi: 10.1016/0005-2760(77)90194-1.
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J Biochem. 1977 Jun;81(6):1639-49. doi: 10.1093/oxfordjournals.jbchem.a131623.
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