S-腺苷-L-甲硫氨酸的（S,S）-和（R,S）-形式的高效液相色谱分离。
High-performance liquid chromatography separation of the (S,S)- and (R,S)-forms of S-adenosyl-L-methionine.
作者信息
Zhang Jianyu, Klinman Judith P
机构信息
Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA.
Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
出版信息
Anal Biochem. 2015 May 1;476:81-3. doi: 10.1016/j.ab.2015.02.004. Epub 2015 Feb 11.
Abstract
S-adenosyl-l-methionine (AdoMet), an important biological cofactor, exists in two chiral forms, (S,S)- and (R,S)-, only the former of which is biologically active. Here, we have developed a chromatographic method to obtain pure (S,S)-AdoMet using a single C18 column.
摘要
S-腺苷-L-甲硫氨酸（AdoMet）是一种重要的生物辅因子，以两种手性形式存在，即（S,S）-型和（R,S）-型，其中只有前者具有生物活性。在此，我们开发了一种色谱方法，使用单一的C18柱来获得纯的（S,S）-AdoMet。
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