Weber L, Meigel W N, Rauterberg J
Arch Dermatol Res (1975). 1977 May 27;258(3):251-7. doi: 10.1007/BF00561127.
A sodium dodecylsulfate-polyacrylamide electrophoretic method, which in contrast to other biochemical procedures, e.g. differential salt precipitation or ion exchange chromatography and molecular sieve chromatography, is applicable to smallest amounts of protein, is shown to be suitable for the determination of the collagen types from small skin samples, such as routine skin biopsies. After urea extraction, the tissue samples are cleaved with cyanogen bromide. The resulting CNBr peptides derived from the different alpha-chains are resolved in 12% SDS-polyacrylamide gels. Densitometric profiles of the gel electrophoretic patterns correspond to the collagen type content of the tissue specimens. Comparing fetal to adult skin, the higher content of type III collagen in the case of fetal skin can be demonstrated.
一种十二烷基硫酸钠-聚丙烯酰胺电泳方法，与其他生化方法（如分级盐沉淀、离子交换色谱法和分子筛色谱法）不同，它适用于极少量的蛋白质，已证明该方法适用于从小皮肤样本（如常规皮肤活检样本）中测定胶原类型。尿素提取后，用溴化氰裂解组织样本。来自不同α链的所得溴化氰肽在12% SDS-聚丙烯酰胺凝胶中分离。凝胶电泳图谱的光密度扫描图与组织标本中的胶原类型含量相对应。比较胎儿皮肤和成人皮肤，可以证明胎儿皮肤中III型胶原含量较高。
