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用于从鱼匀浆中筛选沙门氏菌的富集培养基。

Enrichment medium for selection of Salmonella from fish homogenate.

作者信息

Raj H

出版信息

Appl Microbiol. 1966 Jan;14(1):12-20. doi: 10.1128/am.14.1.12-20.1966.

Abstract

A new liquid medium, called "dulcitol selenite enrichment," has been developed for the detection and enumeration of Salmonella in foods. The medium is not only highly selective in enriching Salmonella and inhibiting completely or appreciably other extraneous organisms commonly found in seafoods, but is also highly sensitive in recovering as low as 2 to 7 cells of Salmonella, even in the presence of large numbers (10(4) to 10(6) cells) of mixed flora common to these foods. The addition of seafood material does not seem to interfere with the sensitivity, selectivity, or productivity of the medium. Even physiologically debilitated cells of Salmonella were enriched well enough in this medium to be detected easily.

摘要

一种名为“杜氏糖醇亚硒酸盐增菌液”的新型液体培养基已被开发出来,用于检测和计数食品中的沙门氏菌。该培养基不仅在富集沙门氏菌方面具有高度选择性,能完全或显著抑制海鲜中常见的其他外来微生物,而且在回收低至2至7个沙门氏菌细胞时也具有高度敏感性,即使在存在大量(10⁴至10⁶个细胞)这些食品中常见的混合菌群的情况下也是如此。添加海鲜材料似乎不会干扰该培养基的敏感性、选择性或增殖能力。即使是生理状态衰弱的沙门氏菌细胞,在这种培养基中也能得到充分富集,从而易于检测。

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