盘基网柄菌核糖体DNA由包含5S和36S编码区的非染色体回文二聚体组成。
Dictyostelium rDNA consists of non-chromosomal palindromic dimers containing 5S and 36S coding regions.
作者信息
Cockburn A F, Taylor W C, Firtel R A
出版信息
Chromosoma. 1978 Dec 21;70(1):19-29. doi: 10.1007/BF00292212.
PMID:738167
Abstract
Restriction enzyme mapping of snap-back DNA has been used to show that the ribosomal DNA (rRNA genes and spacers) from Dictyostelium discoideum exists as 88 kb (kb=1,000 base pairs) linear palindromic dimers. Analysis of the mobility of total cell DNA in 0.15% agarose gels indicates that the majority of the rDNA is not covalently attached to chromosomal DNA.
摘要
通过对回折DNA进行限制性酶切图谱分析，已证明盘基网柄菌的核糖体DNA（rRNA基因和间隔区）以88千碱基对（kb = 1000个碱基对）的线性回文二聚体形式存在。对0.15%琼脂糖凝胶中总细胞DNA迁移率的分析表明，大多数rDNA并非共价连接到染色体DNA上。
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