Shim J S, Tolan J W, Fink D W
J Pharm Sci. 1980 May;69(3):275-9. doi: 10.1002/jps.2600690308.
Fluorescence spectroscopy was applied to the development of sensitive analytical methods for the determination of thiazole and several congeners that contain substituted thiazole rings. Treatment to yield thionine, previously used spectrophotometrically to measure thiazole and fluorometrically only for sulfur determinations in inorganic systems, is further characterized and illustrated with the determination of the antibiotic thiopeptin. This method is selective for submicrogram quantities of thiazole rings in the presence of fused-ring derivatives and reduced analogs. It has a precision of +/- 2% RSD (n = 11) at the 15-ng/ml thiazole concentration level with a signal-to-noise ratio of 3:1. For thiopeptin, this method has an accuracy of 5% mean relative error (n = 8) over the 5--20-ppm range in medicated feed.
荧光光谱法被用于开发灵敏的分析方法，以测定噻唑及几种含有取代噻唑环的同系物。之前用于分光光度法测定噻唑以及仅用于无机体系中硫的荧光测定的生成硫堇的处理方法，通过抗生素硫肽菌素的测定得到了进一步表征和说明。该方法在稠环衍生物和还原类似物存在的情况下，对亚微克量的噻唑环具有选择性。在噻唑浓度为15 ng/ml时，其精密度为±2%相对标准偏差（n = 11），信噪比为3:1。对于硫肽菌素，该方法在加药饲料中5-20 ppm范围内的平均相对误差为5%（n = 8）。
