MacDonald S A, Willson C G, Chorev M, Vernacchia F S, Goodman M
J Med Chem. 1980 Apr;23(4):413-20. doi: 10.1021/jm00178a012.
A series of analogues designed to assess the importance of the amide bond in the dipeptide sweetener L-aspartyl-L-phenylalanine methyl ester has been synthesized and tested. The peptide bond was methylated, replaced by an ester bond, or reversed. all of these modifications produced compounds that did not have a sweet taste. We conclude that the steric, electronic, and directional characteristics of the amide bond are essential for biological activity in the dipeptide sweeteners.
