Kent L, Malemud C J, Moskowitz R W
Prostaglandins. 1980 Mar;19(3):391-406. doi: 10.1016/0090-6980(80)90073-8.
The effects of two unsaturated fatty acids, protaglandin E2, thromboxane B2 (TxB2) and 2 analogs of PG endoperoxide on monolayer cultures of rabbit articular chondrocytes have been studied. Arachidonic and linoleic acids had no effect on either DNA or sulfated-glycosaminoglycan biosynthesis, while 13,14 dihydro-PGE2 and PGE2 markedly inhibited the former. Two epoxymethano analogs of endoperoxide PGH2 (Em-;pgh2) at concentrations of 2.5 and 24 microgram/ml stimulated cell proliferation while reducing 35SO4 incorporation. By contrast, Em-PGH2 at lower concentrations (0.25 - 250 ng/ml) inhibited DNA synthesis in a dose-dependent manner. TxB2 at 2.5 microgram/ml did not alter cellular proliferation. At lower concentrations, 2.5 and 25 ng/ml, TxB2 significantly stimulated sulfated-glycosaminoglycan biosynthesis in at least one of the chondrocyte populations tested. The results also demonstrated marked differences in the effects of TxB2 and the Em-PGH2 analogs on the partitioning of newly synthesized sulfated-proteoglycan between the cells and medium of these cell cultures.
研究了两种不饱和脂肪酸、前列腺素E2、血栓素B2（TxB2）以及PG内过氧化物的2种类似物对兔关节软骨细胞单层培养物的影响。花生四烯酸和亚油酸对DNA或硫酸化糖胺聚糖的生物合成均无影响，而13,14-二氢前列腺素E2和前列腺素E2则显著抑制前者。内过氧化物PGH2的两种环氧甲叉类似物（Em-PGH2）在浓度为2.5和24微克/毫升时刺激细胞增殖，同时减少35SO4掺入。相比之下，较低浓度（0.25 - 250纳克/毫升）的Em-PGH2以剂量依赖方式抑制DNA合成。2.5微克/毫升的TxB2不改变细胞增殖。在较低浓度2.5和25纳克/毫升时，TxB2在至少一种受试软骨细胞群体中显著刺激硫酸化糖胺聚糖的生物合成。结果还表明，TxB2和Em-PGH2类似物对这些细胞培养物的细胞与培养基之间新合成的硫酸化蛋白聚糖分配的影响存在显著差异。
