Kornilaeva G V, Men'shikh L K, Akishpaeva O T, Bukrinskaia A G, Zhdanov V M
Vopr Virusol. 1980 Mar-Apr(2):141-7.
Adsorption and penetration into chick fibroblast cells of WSN virus with completely cleaved hemagglutinin precursor (virus grown in chick embryos), partially cleaved hemagglutinin precursor (virus grown in chick fibroblasts), and virus treated with trypsin added to infected cells or virions were studied. Treatment with trypsin in both instances resulted in complete cleavage of hemagglutinin precursor and in more than 100-fold increase of the infectious activity. Chick embryo-grown virus adsorbed in the same manner as the culture-grown virus not treated with trypsin, but penetrated into the cells 2--3 times more effectively. The culture-grown virus after treatment with trypsin adsorbed better but penetrated in the same way as the original culture-grown virus. Treatment of the infected cells with trypsin led to more effective penetration with unchanged adsorption. The experimental results suggest that cleavage of hemagglutinin precursor is essential both for adsorption and penetration. Depending on the method of treatment, the effectiveness of one or the other process is increased.
研究了具有完全裂解血凝素前体的WSN病毒（在鸡胚中生长的病毒）、部分裂解血凝素前体的病毒（在鸡成纤维细胞中生长的病毒）以及添加到感染细胞或病毒粒子中并用胰蛋白酶处理的病毒对鸡成纤维细胞的吸附和穿透情况。在这两种情况下，用胰蛋白酶处理都会导致血凝素前体的完全裂解，并使感染活性增加100倍以上。鸡胚生长的病毒与未用胰蛋白酶处理的培养生长病毒以相同方式吸附，但穿透细胞的效率提高了2至3倍。用胰蛋白酶处理后的培养生长病毒吸附性更好，但穿透方式与原始培养生长病毒相同。用胰蛋白酶处理感染细胞导致穿透更有效而吸附不变。实验结果表明，血凝素前体的裂解对于吸附和穿透都是必不可少的。根据处理方法，一个或另一个过程的有效性会增加。
