[从感染流感病毒的鸡成纤维细胞中分离细胞核]
[Isolation of nuclei from influenza virus infected chick fibroblast cells].
作者信息
Petrik I U, Sklianskaia E I, Varich N L
出版信息
Vopr Virusol. 1980 Mar-Apr(2):157-61.
PMID:7385787
Abstract
Three methods of nuclei isolation from influenza virus-infected chick fibroblast cells are characterized comparatively. The purity of the nuclear preparations was evaluated by hemagglutinin determination in them in hemagglutination test or hemagglutinin and membrane protein determination by polyacrylamide gel electrophoresis of labeled proteins. The purest nuclei were prepared by the method using citric acid--MgCl2--triton X-100.
摘要
对从感染流感病毒的鸡成纤维细胞中分离细胞核的三种方法进行了比较表征。通过血凝试验中对细胞核制剂进行血凝素测定，或通过对标记蛋白质进行聚丙烯酰胺凝胶电泳来测定血凝素和膜蛋白，从而评估细胞核制剂的纯度。使用柠檬酸-MgCl2-曲拉通X-100的方法制备出了纯度最高的细胞核。
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