Stuart J M, Postlethwaite A E, Townes A S, Kang A H
Am J Med. 1980 Jul;69(1):13-8. doi: 10.1016/0002-9343(80)90494-5.
Peripheral blood mononuclear cells from patients with rheumatoid arthritis, gout, ankylosing spondylitis and degenerative joint disease were cultured in the presence of native types I, II and III collagens and alpha chains from each of these types of collagen. The culture supernatant fluids were harvested and assayed for lymphocyte-derived chemotatic factor for monocytes. Reactions to one or more of the native collagens was found in 50 per cent (10 of 20) of the patients with rheumatoid arthritis, 20 per cent (two of 10) of the patients with gout and ankylosing spondylitis but in none of the 10 patients with degenerative joint disease or in normal subjects. Reaction to one or more alpha chains was found in 90 per cent (18 of 20) of the patients with rheumatoid arthritis, 60 per cent (six of 10) of the patients with gout, 50 per cent (five of 10) of the patients with ankylosing spondylitis, 30 per cent (three of 10) of the patients with degenerative joint disease and in 10 per cent of the normal subjects (one of 10). All the reactions were quantiatively stronger in patients with rheumatoid arthritis. These results indicate that patients with rheumatoid arthritis have cell-mediated immunity to homologous native and denatured collagens but that the reaction is not specific for rheumatoid arthritis. Some patients with gout, ankylosing spondylitis and degenerative joint disease also have low levels of immunity.
将类风湿性关节炎、痛风、强直性脊柱炎和退行性关节病患者的外周血单核细胞，在天然I型、II型和III型胶原蛋白以及这些类型胶原蛋白的α链存在的情况下进行培养。收集培养上清液，并检测单核细胞的淋巴细胞衍生趋化因子。在50%（20例中的10例）的类风湿性关节炎患者、20%（10例中的2例）的痛风和强直性脊柱炎患者中发现对一种或多种天然胶原蛋白有反应，但在10例退行性关节病患者或正常受试者中均未发现。在90%（20例中的18例）的类风湿性关节炎患者、60%（10例中的6例）的痛风患者、50%（10例中的5例）的强直性脊柱炎患者、30%（10例中的3例）的退行性关节病患者以及10%的正常受试者（10例中的1例）中发现对一种或多种α链有反应。类风湿性关节炎患者的所有反应在数量上都更强。这些结果表明，类风湿性关节炎患者对同源天然和变性胶原蛋白具有细胞介导的免疫，但这种反应并非类风湿性关节炎所特有的。一些痛风、强直性脊柱炎和退行性关节病患者也有低水平的免疫。
