Hocking A D, Pitt J I
Appl Environ Microbiol. 1980 Mar;39(3):488-92. doi: 10.1128/aem.39.3.488-492.1980.
A low water activity (alpha omega) medium (0.95 alpha omega) containing 18% (wt/wt) glycerol and 2 micrograms of dichloran per ml was developed for enumerating the fungal flora of dried and semidried foods. The medium, designated DG18, was shown to be significantly better than Christensen malt salt agar when both media were tested with foodstuffs and with pure culture inocula. The need for a medium of reduced alpha omega for enumerating xerophilic fungi from low-moisture foods was demonstrated by comparing fungal counts obtained on both high-alpha omega and low-alpha omega media.
开发了一种低水分活度(αω)培养基(αω为0.95),其含有18%(重量/重量)甘油且每毫升含有2微克二氯苯胺,用于对干制和半干制食品中的真菌菌群进行计数。这种被命名为DG18的培养基在与食品及纯培养接种物一起进行测试时,显示出明显优于克里斯滕森麦芽盐琼脂。通过比较在高αω和低αω培养基上获得的真菌计数,证明了需要一种降低αω的培养基来对低水分食品中的嗜旱真菌进行计数。