酶活性位点基团pK值的图形测定。钟形曲线分析。
Graphical determination of pK values of the active-site groups of enzymes. An analysis of the bell-shaped curves.
作者信息
Parkash O, Bhatia I S
出版信息
Biochem J. 1980 Mar 1;185(3):609-10. doi: 10.1042/bj1850609.
Abstract
Descriptions are given for the graphical determination of molecular pK values from the curves of logV and p(Km/V) against pH. The arguments are based on the model dissociation equilibria LAL in equilibrium (AL + LA) in equilibrium A, where A denotes a molecule having two binding sites for a ligand L.
摘要
给出了根据logV和p(Km/V)对pH的曲线通过图形确定分子pK值的方法。这些论证基于配体L在平衡态（AL + LA）处于平衡态A时的模型解离平衡，其中A表示具有两个配体L结合位点的分子。
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