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腺病毒病毒相关RNAI在瞬时表达试验中控制翻译的机制。

A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay.

作者信息

Akusjärvi G, Svensson C, Nygård O

出版信息

Mol Cell Biol. 1987 Jan;7(1):549-51. doi: 10.1128/mcb.7.1.549-551.1987.

DOI:10.1128/mcb.7.1.549-551.1987
PMID:2436038
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365102/
Abstract

The mechanism by which adenovirus virus-associated RNAI stimulates translational efficiency in a transient-expression assay in 293 cells was investigated. We showed that DNA transfection leads to activation of a protein kinase that phosphorylates the alpha subunit of eucaryotic initiation factor 2 and, as a consequence, inhibition of polypeptide chain initiation. Cotransfection of a plasmid encoding adenovirus type 2 virus-associated RNAI recovered the translational capacity by preventing activation of the kinase.

摘要

研究了腺病毒相关RNAI在293细胞的瞬时表达试验中刺激翻译效率的机制。我们发现DNA转染会导致一种蛋白激酶的激活，该激酶会使真核起始因子2的α亚基磷酸化，从而抑制多肽链的起始。共转染编码2型腺病毒相关RNAI的质粒可通过阻止激酶的激活来恢复翻译能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/365102/52e1a2e2b64f/molcellb00073-0570-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/365102/47dbd1b0c566/molcellb00073-0569-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/365102/355bd877c4d1/molcellb00073-0570-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/365102/52e1a2e2b64f/molcellb00073-0570-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/365102/47dbd1b0c566/molcellb00073-0569-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/365102/355bd877c4d1/molcellb00073-0570-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/365102/52e1a2e2b64f/molcellb00073-0570-b.jpg

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A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay.腺病毒病毒相关RNAI在瞬时表达试验中控制翻译的机制。
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本文引用的文献

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Transcriptional control regions of the adenovirus VAI RNA gene.腺病毒VAI RNA基因的转录控制区域。
Cell. 1980 Nov;22(2 Pt 2):405-13. doi: 10.1016/0092-8674(80)90351-7.
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Selective stimulation of translational expression by Alu RNA.Alu RNA对翻译表达的选择性刺激。
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Potential Alu function: regulation of the activity of double-stranded RNA-activated kinase PKR.潜在的Alu功能：双链RNA激活激酶PKR活性的调节。
Mol Cell Biol. 1998 Jan;18(1):58-68. doi: 10.1128/MCB.18.1.58.
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Comparative analysis of the structure and function of adenovirus virus-associated RNAs.腺病毒病毒相关RNA的结构与功能比较分析
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Adenovirus mutants with DNA sequence perturbations in the intragenic promoter of VAI RNA gene allow the enhanced transcription of VAII RNA gene in HeLa cells.在VAI RNA基因的基因内启动子中具有DNA序列扰动的腺病毒突变体能够增强HeLa细胞中VAII RNA基因的转录。
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