[标记皮质醇掺入不同类型的结缔组织]

[Incorporation of labeled cortisol into different types of connective tissue].

作者信息

Torbenko V P, Kasavina B S, Ukhina T V

出版信息

Biull Eksp Biol Med. 1980 Apr;89(4):481-3.

Abstract

The properties of early (BFUen) and late (CFUen) erythropoietin-independent progenitors were studied in bone marrow plasma clot cultures of mice. Syngeneic serum was used as a stimulant of colony formation. It was revealed that serum from polycytemic mice did not alter the efficacy of cloning the erythroid progenitors. Varying effects produced on erythropoiesis in vivo did not significantly change the number of CFUen and BFUen, with the exception of posttransfusion polycytemia where the number of CFUen showed a statistically significant rise. Assessment of radiosensitivity and proliferative activity of the erythroid progenitors showed that for CFUen D0=206 rad and n=1.3; for BFUen D0=118 rad and n=1.4; the proportion of proliferating CFUen was 42% +/- 12.5 and that of BFUen 46% +/- 4.5. The data obtained confirm the hypothesis of the erythropoietin-independent nature of the erythroid progenitors expressed under the culture conditions used.

摘要

在小鼠骨髓血浆凝块培养物中研究了早期（爆式红系集落形成单位，BFUen）和晚期（红系集落形成单位，CFUen）非促红细胞生成素依赖性祖细胞的特性。同基因血清用作集落形成的刺激物。结果显示，来自红细胞增多症小鼠的血清并未改变红系祖细胞的克隆效率。除输血后红细胞增多症（此时CFUen数量出现统计学显著升高）外，体内对红细胞生成产生的不同影响并未显著改变CFUen和BFUen的数量。对红系祖细胞的放射敏感性和增殖活性评估显示，CFUen的D0=206拉德，n=1.3；BFUen的D0=118拉德，n=1.4；增殖性CFUen的比例为42%±12.5%，BFUen的比例为46%±4.5%。所获数据证实了在所采用培养条件下表达的红系祖细胞非促红细胞生成素依赖性性质的假说。