Chinn D M, Jennison T A, Crouch D J, Peat M A, Thatcher G W
Clin Chem. 1980 Jul;26(8):1201-4.
We describe a gas chromatographic-chemical-ionization mass spectrometric procedure for determining concentrations of the tricyclic antidepressant drugs and their demethylated metabolites in plasma. The procedure requires 2.0 mL of plasma or serum and involves internal standard addition, extraction with a mixed organic solvent, and separation on a column packed with 0.5% potassium hydroxide and 2% Carbowax 20M. Between-assay and within-assay (n = 10) CVs were less than 11.0%. The procedure has been applied to several interesting cases, three of which are summarized.
我们描述了一种气相色谱 - 化学电离质谱法，用于测定血浆中三环类抗抑郁药物及其去甲基代谢物的浓度。该方法需要2.0 mL血浆或血清，包括添加内标、用混合有机溶剂萃取以及在填充有0.5%氢氧化钾和2%聚乙二醇20M的柱上进行分离。批间和批内（n = 10）变异系数均小于11.0%。该方法已应用于几个有趣的病例，其中三个病例进行了总结。
