Ambler J, Rodgers M
Clin Chem. 1980 Jul;26(8):1221-3.
Two new non-barbiturate buffers have been formulated for serum protein electrophoresis on cellulose acetate membranes. Both buffers give five distrinct fractions and are suitable for all systems, but have been primarily designed to meet the needs of the Gelman "Sepratek" system. One buffer is a tris(hydroxymethyl)aminomethane (Tris)/hippurate formulation; the other is composed of Tris/N-[tris(hydroxymethyl)methyl]glycine (Tricine)/sodium chloride/sodium salicylate. Both buffers allow reliable quantitation of protein fractions, giving results that correlated well with those obtained from barbiturate separations. The major advantage of these buffers is that they do not contain drugs of abuse.
已配制出两种新型非巴比妥缓冲液，用于醋酸纤维素膜上的血清蛋白电泳。两种缓冲液都能给出五个清晰的组分，适用于所有系统，但主要是为满足吉尔曼“Sepratek”系统的需求而设计的。一种缓冲液是三（羟甲基）氨基甲烷（Tris）/马尿酸盐配方；另一种由Tris/N-[三（羟甲基）甲基]甘氨酸（Tricine）/氯化钠/水杨酸钠组成。两种缓冲液都能对蛋白质组分进行可靠的定量分析，所得结果与巴比妥分离法得到的结果相关性良好。这些缓冲液的主要优点是它们不含违禁药物。
