Mathis D F, Budd R D
Clin Toxicol. 1980 Apr;16(2):181-8. doi: 10.3109/15563658008989936.
A procedure has been developed for the TLC and GLC analysis of codeine and morphine derivatives in urine in cases where there is too much interference for TLC analysis as free drugs. Urine is hydrolyzed, then split into two fractions. One fraction is extracted with a polar solvent, concentrated, and acetylated. An aliquot is injected in a 3% OV-17 column and the rest is spotted on a plate developed in hexane:chloroform:diethylamine (50:30:6). The other fraction is ethylated (converting morphine to ethylmorphine), extracted with a nonpolar solvent, concentrated, acetylated, concentrated, and then spotted on a plate developed in hexane:n-butanol:acetonitrile:diethylamine (80:5:5:10). Codeine and morphine can be detected without interference at concentrations as low as 0.3 micrograms/mL.
已开发出一种用于尿中可待因和吗啡衍生物的薄层色谱（TLC）和气相色谱（GLC）分析的方法，适用于因游离药物干扰过多而无法进行TLC分析的情况。尿液先进行水解，然后分成两部分。一部分用极性溶剂萃取、浓缩并乙酰化。取一份注入3% OV - 17柱，其余点样于以己烷：氯仿：二乙胺（50:30:6）展开的薄板上。另一部分进行乙基化（将吗啡转化为乙基吗啡），用非极性溶剂萃取、浓缩、乙酰化、再浓缩，然后点样于以己烷：正丁醇：乙腈：二乙胺（80:5:5:10）展开的薄板上。可待因和吗啡在低至0.3微克/毫升的浓度下即可无干扰地检测出来。
