Yeomans N D, Millar S J
Dig Dis Sci. 1980 Apr;25(4):295-301. doi: 10.1007/BF01308521.
Measurement of gastric mucus production in vivo poses several problems. We therefore used organ culture of rat antrum to measure mucosal synthesis and secretion of glycoprotein. Tissue maintained good viability for 20 hr. Glycoprotein synthesis rate, assessed by incorporation of [3H]fucose and [3H]glucosamine, was constant during 20 hr. [3H]Fucose incorporation increased in proportion to increasing [fucose] in medium until the latter reached 10(-3) M, then the slope decreased. Cycloheximide abolished [3H]fucose incorporation into glycoprotein. Two methods for measuring secretion gave parallel results: prelabeled glycoprotein was secreted linearly, with 60% of pulse-labeled tissue glycoprotein appearing in medium in 20 hr. The data suggest that (1) antral mucous cells contain a high-concentration pool of endogenous fucose, but possibly only a small-capacity pool of preformed apoglycoprotein, (2) synthesis and secretion of glycoprotein can be successfully examined with organ culture, and (3) secretion in vitro occurs at a steady, slow rate.
体内胃黏液生成的测量存在几个问题。因此，我们采用大鼠胃窦的器官培养来测量糖蛋白的黏膜合成与分泌。组织在20小时内保持良好的活力。通过掺入[3H]岩藻糖和[3H]葡糖胺评估的糖蛋白合成速率在20小时内保持恒定。[3H]岩藻糖的掺入量与培养基中[岩藻糖]浓度的增加成比例增加，直至后者达到10(-3) M，然后斜率下降。放线菌酮消除了[3H]岩藻糖掺入糖蛋白的过程。两种测量分泌的方法得出了平行的结果：预先标记的糖蛋白呈线性分泌，脉冲标记的组织糖蛋白在20小时内有60%出现在培养基中。这些数据表明：(1)胃窦黏液细胞含有高浓度的内源性岩藻糖池，但可能仅含有少量预先形成的脱辅基糖蛋白池；(2)利用器官培养可以成功检测糖蛋白的合成与分泌；(3)体外分泌以稳定、缓慢的速率发生。
