溃疡性结肠炎患者结肠组织的体外黏液糖蛋白生成
In vitro mucus glycoprotein production by colonic tissue from patients with ulcerative colitis.
作者信息
Cope G F, Heatley R V, Kelleher J, Axon A T
机构信息
Department of Medicine, St. James's University Hospital, Leeds.
出版信息
Gut. 1988 Feb;29(2):229-34. doi: 10.1136/gut.29.2.229.
Abstract
Colonic mucus production was measured in vitro by means of incorporation of tritiated glucosamine using biopsy material from patients with ulcerative colitis and compared with data from patients with Crohn's disease, colonic carcinoma, colonic polyps and patients with apparently normal colonic mucosae. Mucus production was significantly decreased (p less than 0.03) in all patients with ulcerative colitis, and in particular in patients with inactive disease when compared with normal subjects. In patients with active disease mucus production was not significantly different from normal subjects. The radiolabelled material was characterised by gel filtration and ion exchange liquid chromatography as mainly high molecular weight glycoproteins. These results indicate that the quantitative character of colonic mucus is abnormal in inactive ulcerative colitis.
摘要
通过使用来自溃疡性结肠炎患者的活检材料掺入氚标记的葡糖胺，在体外测量结肠黏液分泌，并与克罗恩病、结肠癌、结肠息肉患者以及结肠黏膜明显正常的患者的数据进行比较。与正常受试者相比，所有溃疡性结肠炎患者的黏液分泌均显著减少（p<0.03），尤其是无活动期疾病的患者。活动期疾病患者的黏液分泌与正常受试者无显著差异。通过凝胶过滤和离子交换液相色谱法对放射性标记物质进行表征，结果表明其主要为高分子量糖蛋白。这些结果表明，非活动期溃疡性结肠炎患者结肠黏液的定量特征异常。
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