Shearman P J, Longenecker B M
Int J Cancer. 1980 Mar 15;25(3):363-9. doi: 10.1002/ijc.2910250310.
The Marek's Disease virus-transformed, non-producer, lymphoma cell line, MDCC-RPI, was selected by sequential transplantation to produce a highly malignant variant, MDCC-ALI. This is evidenced gy an 18-fold decrease in LD50. The new cell line has an increased ability to form metastatic lesions in a distribution which mimics the natural disease. Further selections for organ-specific metastasis were undertaken with the isolation of two cell lines, MDCC-AL2, selected for liver metastasis, and MDCC-AL3, selected for ovary metastasis. In vivo studies show that the selection was unsuccessful in the case of the ovary but successful in the case of the liver. Two assays were developed, utilizing the chick embryo and intravenous injection of lymphoma variants. One measures liver-specific metastasis by the enumeration of tumor foci on the embryonic liver. The second, chorioallantoic membrane (CAM) focus formation, correlates with the virulence of the injected lymphoma cells. The liver-selected tumour variant cells form more liver foci than any other tumour variant cells. The genetic background of the embryo does not affect formation of liver foci. Resistance to CAM focus formation correlates with major histocompatibility complex associated resistance to MD.
通过连续传代移植，选择了马立克氏病病毒转化的非生产性淋巴瘤细胞系MDCC - RPI，以产生高度恶性的变体MDCC - ALI。这通过半数致死剂量（LD50）降低了18倍得到证明。新的细胞系形成转移灶的能力增强，其分布模拟自然疾病。通过分离两个细胞系进行了针对器官特异性转移的进一步选择，即选择用于肝转移的MDCC - AL2细胞系和选择用于卵巢转移的MDCC - AL3细胞系。体内研究表明，卵巢转移的选择未成功，但肝转移的选择成功。利用鸡胚和静脉注射淋巴瘤变体开发了两种检测方法。一种通过计数胚胎肝脏上的肿瘤灶来测量肝特异性转移。第二种，绒毛尿囊膜（CAM）灶形成，与注射的淋巴瘤细胞的毒力相关。肝选择的肿瘤变体细胞比任何其他肿瘤变体细胞形成更多的肝灶。胚胎的遗传背景不影响肝灶的形成。对CAM灶形成的抗性与主要组织相容性复合体相关的对马立克氏病的抗性相关。
