McGuire E J, Mascali J J, Grady S R, Nicolson G L
Clin Exp Metastasis. 1984 Jul-Sep;2(3):213-22. doi: 10.1007/BF00132928.
Metastatic variant sublines of the murine RAW117 large cell lymphoma or lymphosarcoma have been established in vitro by sequential cycles of harvesting of liver tumor nodules after intravenous inoculation of tumor cell suspensions into syngeneic BALB/c mice. After five and ten in vivo selections for liver colonization, variant sublines RAW117-H5 and -H10, respectively, were established, and these formed significantly more surface liver tumors than the parental RAW117-P line. RAW117 sublines were tested for their abilities to adhere to embryonic mouse liver or brain cells in an in vitro cell-cell adhesion assay. Liver colonizing RAW117-H10 cells adhered with greater selectivity to liver cells than to brain cells. Parental RAW117-P cells were more homotypically adhesive, but they were nonselective in their organ cell adhesion properties. We examined RAW117 cells for the presence of liver cross-reactive antigens using polyclonal xenoantibody preparations directed against embryonic murine liver cells. These antibody preparations block organ-specific homotypic adhesion of embryonic murine liver cells in vitro. The amount of fetal liver antigen(s) expressed on RAW117 sublines correlated with liver colonization potentials (H10 greater than H5 greater than P) in quantitative absorption assays. Treatment of the highly metastatic RAW117-H10 subline with polyclonal anti-embryonic murine liver F(ab')2 or Fab' antibody fragments had no effect on RAW117-H10 cell viability or growth in vitro or in vivo, but inhibited liver colonization (median liver tumor colonies reduced from greater than 200 to 0) and prolonged life expectancy. In contrast, pretreatment of RAW117-H10 cells with polyclonal anti-H-2 did not modify the in vivo biologic properties of these metastatic cells.
通过将肿瘤细胞悬液静脉注射到同基因BALB/c小鼠体内，然后依次循环采集肝肿瘤结节，已在体外建立了小鼠RAW117大细胞淋巴瘤或淋巴肉瘤的转移变异亚系。在对肝脏定植进行5次和10次体内选择后，分别建立了变异亚系RAW117-H5和-H10，与亲代RAW117-P系相比，这些亚系形成的肝脏表面肿瘤明显更多。在体外细胞-细胞黏附试验中，检测了RAW117亚系黏附胚胎小鼠肝细胞或脑细胞的能力。肝脏定植的RAW117-H10细胞对肝细胞的黏附选择性高于对脑细胞的黏附选择性。亲代RAW117-P细胞同型黏附性更强，但它们在器官细胞黏附特性上没有选择性。我们使用针对胚胎小鼠肝细胞的多克隆异种抗体制剂，检测了RAW117细胞中是否存在肝脏交叉反应抗原。这些抗体制剂在体外可阻断胚胎小鼠肝细胞的器官特异性同型黏附。在定量吸收试验中，RAW117亚系上表达的胎儿肝抗原量与肝脏定植潜能相关（H10大于H5大于P）。用多克隆抗胚胎小鼠肝脏F(ab')2或Fab'抗体片段处理高转移性RAW117-H10亚系，对RAW117-H10细胞在体外或体内的活力或生长没有影响，但抑制了肝脏定植（肝脏肿瘤集落中位数从大于200减少到0）并延长了预期寿命。相比之下，用多克隆抗H-2预处理RAW117-H10细胞并没有改变这些转移细胞的体内生物学特性。
