• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

蚊子(伪盾纹伊蚊)细胞系(Mos. 61)中病毒样颗粒的初步特性分析。

Preliminary characterization of virus-like particles in a mosquito (Aedes pseudoscutellaris) cell line (Mos. 61).

作者信息

Gorziglia M, Botero L, Gil F, Esparza J

出版信息

Intervirology. 1980;13(4):232-40. doi: 10.1159/000149130.

DOI:10.1159/000149130
PMID:7390733
Abstract

Electron microscopic examination of an Aedes pseudoscutellaris mosquito cell line (Mos. 61) revealed the presence of a large number of virus-like particles (VLP) in the cytoplasm of approximately 10% of the cells. These particles have a diameter of 36 nm, do not contain a lipid envelope, and have a buoyant density of 1.40 g/ml in CsCl. VLP contain DNA which appears to be single-stranded by SU nuclease assay. PAGE of the VLP demonstrates the presence of four structural polypeptides with molecular weights of 52K, 70K, 75K and 100K daltons, the smaller one being the majro polypeptide species and accounting for 71% of the the total protein mass. The A. pseudoscutellaris VLP appear to share some, but not all, of the characteristics of the genus Densovirus, family Parvoviridae. Their possible biologic importance and taxonomic location are still unclear.

摘要

对伪盾伊蚊蚊子细胞系(Mos. 61)进行电子显微镜检查发现,约10%的细胞胞质中存在大量病毒样颗粒(VLP)。这些颗粒直径为36纳米,不含脂质包膜,在氯化铯中的浮力密度为1.40克/毫升。VLP含有DNA,经SU核酸酶测定似乎为单链。VLP的聚丙烯酰胺凝胶电泳显示存在四种结构多肽,分子量分别为52K、70K、75K和100K道尔顿,较小的一种是主要的多肽种类,占总蛋白质量的71%。伪盾伊蚊VLP似乎具有细小病毒科浓核病毒属的一些但并非全部特征。它们可能的生物学重要性和分类地位仍不清楚。

相似文献

1
Preliminary characterization of virus-like particles in a mosquito (Aedes pseudoscutellaris) cell line (Mos. 61).蚊子(伪盾纹伊蚊)细胞系(Mos. 61)中病毒样颗粒的初步特性分析。
Intervirology. 1980;13(4):232-40. doi: 10.1159/000149130.
2
Production and characterization of the cores of the "R" strain of mosquito iridescent virus.蚊虫虹彩病毒“R”株核心的产生与特性分析
Virology. 1975 Apr;64(2):430-7. doi: 10.1016/0042-6822(75)90120-8.
3
Biochemical and biophysical properties of two strains of mosquito iridescent virus.两种蚊虫虹彩病毒株的生化及生物物理特性
Virology. 1973 Mar;52(1):72-80. doi: 10.1016/0042-6822(73)90399-1.
4
A comparison of buoyant density and polypeptides of Drosophila P, C and A viruses.果蝇P病毒、C病毒和A病毒的浮力密度及多肽比较
Intervirology. 1976;7(6):346-50. doi: 10.1159/000149975.
5
A parvo-like virus persistently infecting a C6/36 clone of Aedes albopictus mosquito cell line and pathogenic for Aedes aegypti larvae.
Virus Res. 1993 Aug;29(2):99-114. doi: 10.1016/0168-1702(93)90052-o.
6
[Characteristics of the densonucleosis virus of mosquitoes].
Mikrobiol Zh (1978). 1985 Jan-Feb;47(1):68-73.
7
Characterization of the top component of the "T" strain of mosquito iridescent virus.
Virology. 1977 Jan;76(1):426-8. doi: 10.1016/0042-6822(77)90315-4.
8
Queensland fruit fly virus, a probable member of the Picornaviridae.昆士兰果蝇病毒,可能是小核糖核酸病毒科的成员。
Arch Virol. 1988;100(1-2):61-74. doi: 10.1007/BF01310908.
9
Expansion of family Reoviridae to include nine-segmented dsRNA viruses: isolation and characterization of a new virus designated Aedes pseudoscutellaris reovirus assigned to a proposed genus (Dinovernavirus).呼肠孤病毒科的扩展以纳入九节段双链RNA病毒:一种新病毒的分离与鉴定,该病毒被命名为伪盾伊蚊呼肠孤病毒,被归入一个提议的属(恐龙病毒属)
Virology. 2005 Dec 20;343(2):212-23. doi: 10.1016/j.virol.2005.08.028. Epub 2005 Sep 19.
10
Electron microscope study of mosquito densonucleosis virus maturation.蚊子浓核症病毒成熟过程的电子显微镜研究。
Acta Virol. 1979 Mar;23(2):170-2.

引用本文的文献

1
Detection by electron microscopy of endogenous viruses in Aedes krombeini (H.) cell line.通过电子显微镜检测克氏伊蚊(H.)细胞系中的内源性病毒。
In Vitro Cell Dev Biol Anim. 1994 Mar;30A(3):142-4. doi: 10.1007/BF02631435.
2
Characterization of invertebrate cell lines. IV. Isozyme analyses of dipteran and acarine cell lines.无脊椎动物细胞系的特性研究。IV. 双翅目和蜱螨目细胞系的同工酶分析。
In Vitro. 1982 Apr;18(4):347-50. doi: 10.1007/BF02796333.