Brown S E, Knudson D L
In Vitro. 1982 Apr;18(4):347-50. doi: 10.1007/BF02796333.
Cell extracts of five mosquito cell lines and a tick cell line were examined for four cellular isozymes using a cellulose-acetate electrophoretic technique. This method distinguished the cell lines that were derived from the different species. Intraspecies distinctions were not made using the cell lines tested; the significance of this finding is discussed. The usefulness of this technique in identifying a potentially mislabeled cell line was demonstrated.
使用醋酸纤维素电泳技术,对五种蚊细胞系和一种蜱细胞系的细胞提取物进行了四种细胞同工酶的检测。该方法区分了源自不同物种的细胞系。在所检测的细胞系中未进行种内区分;对这一发现的意义进行了讨论。证明了该技术在鉴定可能被错误标记的细胞系方面的实用性。