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无脊椎动物细胞系的特性研究。IV. 双翅目和蜱螨目细胞系的同工酶分析。

Characterization of invertebrate cell lines. IV. Isozyme analyses of dipteran and acarine cell lines.

作者信息

Brown S E, Knudson D L

出版信息

In Vitro. 1982 Apr;18(4):347-50. doi: 10.1007/BF02796333.

DOI:10.1007/BF02796333
PMID:6126429
Abstract

Cell extracts of five mosquito cell lines and a tick cell line were examined for four cellular isozymes using a cellulose-acetate electrophoretic technique. This method distinguished the cell lines that were derived from the different species. Intraspecies distinctions were not made using the cell lines tested; the significance of this finding is discussed. The usefulness of this technique in identifying a potentially mislabeled cell line was demonstrated.

摘要

使用醋酸纤维素电泳技术,对五种蚊细胞系和一种蜱细胞系的细胞提取物进行了四种细胞同工酶的检测。该方法区分了源自不同物种的细胞系。在所检测的细胞系中未进行种内区分;对这一发现的意义进行了讨论。证明了该技术在鉴定可能被错误标记的细胞系方面的实用性。

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In Vitro. 1982 Apr;18(4):347-50. doi: 10.1007/BF02796333.
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本文引用的文献

1
Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence.仓鼠细胞克隆的多瘤病毒转化——对影响细胞感受态的遗传因素的研究
Virology. 1962 Feb;16:147-51. doi: 10.1016/0042-6822(62)90290-8.
2
THE GROWTH AND MAINTENANCE OF TISSUE-CELL CULTURES IN FREE GAS EXCHANGE WITH THE ATMOSPHERE.与大气进行自由气体交换的组织细胞培养物的生长与维持
Am J Hyg. 1963 Sep;78:173-80. doi: 10.1093/oxfordjournals.aje.a120336.
3
Preliminary characterization of virus-like particles in a mosquito (Aedes pseudoscutellaris) cell line (Mos. 61).
Exp Appl Acarol. 1989 Aug;7(3):219-29. doi: 10.1007/BF01194061.
4
Characterization of a new continuous cell line from the flood water mosquito, Aedes vexans.来自洪水蚊类——骚扰伊蚊的一种新连续细胞系的特性分析
Cytotechnology. 1991 Feb;5(2):147-54. doi: 10.1007/BF00365431.
蚊子(伪盾纹伊蚊)细胞系(Mos. 61)中病毒样颗粒的初步特性分析。
Intervirology. 1980;13(4):232-40. doi: 10.1159/000149130.
4
The growth and serial passage of cell lines from Aedes aegypti (L.) larvae in different media.
J Med Entomol. 1969 Oct;6(4):432-9. doi: 10.1093/jmedent/6.4.432.
5
Growth of arboviruses in arthropod tissue culture.虫媒病毒在节肢动物组织培养中的生长
Adv Virus Res. 1972;17:187-206. doi: 10.1016/s0065-3527(08)60750-2.
6
Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses.分离出对登革热病毒和基孔肯雅病毒敏感的辛格白纹伊蚊细胞克隆。
J Gen Virol. 1978 Sep;40(3):531-44. doi: 10.1099/0022-1317-40-3-531.
7
A method for the isolation and identification of dengue viruses, using mosquito cell cultures.
Am J Trop Med Hyg. 1979 Nov;28(6):1053-9. doi: 10.4269/ajtmh.1979.28.1053.