McGuire E J
J Cell Biol. 1976 Jan;68(1):90-100. doi: 10.1083/jcb.68.1.90.
Studies directed at understanding the molecular basis of liver cell homotypic adhesion are presented. An assay which measures the rate of adhesion of isotopically labeled (32PO4) embryonic chick liver cells to liver cell aggregates, described in a companion paper, has been used to investigate the problem of intercellular adhesive selectivity. Cation requirements, the effects of various inhibitors of metabolism and protein synthesis, of chelators (EDTA and EGTA), and the effects of temperature on liver cell adhesion are reported. Two mechanisms of inhibition of liver intercellular adhesion are suggested. One involves destruction of cell-surface adhesion receptors (sensitivity to proteases); the other is an energy-dependent step which may involve alterations in plasma membrane conformation and/or membrane fluidity. Finally, a model is suggested for liver cell-cell adhesion that incorporates the early tissue selectivity of intercellular adhesion previously reported, followed by a multistep process which leads to histogenic aggregation.
本文介绍了旨在理解肝细胞同型黏附分子基础的研究。在一篇配套论文中描述了一种检测方法，该方法用于测量同位素标记（³²PO₄）的胚胎鸡肝细胞与肝细胞聚集体的黏附速率，已被用于研究细胞间黏附选择性的问题。报告了阳离子需求、各种代谢和蛋白质合成抑制剂、螯合剂（EDTA和EGTA）的作用以及温度对肝细胞黏附的影响。提出了两种抑制肝细胞间黏附的机制。一种涉及细胞表面黏附受体的破坏（对蛋白酶敏感）；另一种是能量依赖步骤，可能涉及质膜构象和/或膜流动性的改变。最后，提出了一个肝细胞-细胞黏附模型，该模型包含先前报道的细胞间黏附的早期组织选择性，随后是一个导致组织发生聚集的多步骤过程。
