Gates R J, Lazarus N R
Lancet. 1977 Dec 17;2(8051):1257-9. doi: 10.1016/s0140-6736(77)92664-2.
Implants of rabbit neonatal pancreas, encased in 'Nucleopore' chambers (0.4 micrometer) reversed streptozotocin-induced diabetes in the rat. Blood-glucose, plasma-insulin, and oral glucose-tolerance test returned to normal. An isolated, perfused, streptozotocin-treated pancreas removed from a diabetic animal did not secrete insulin and removal of implants after 6 weeks from six animals caused all animals to die in hyperglycaemia within 8 days. This shows that the implant did not lead to the re-establishment of endogenous pancreatic function. Implanted diced neonatal pancreas in three chambers removed after 6 weeks secreted glucagon, insulin, and pancreatic polypeptide in vitro. No rejection reactions were seen. Rabbit neonatal pancreatic implants may thus be feasible therapy in insulin-requiring diabetic patients. Implants of other non-syngeneic endocrine cells--i.e., pituitary, thyroid, and ovary--may be useful in other hypoendocrine syndromes.
包裹在“核孔”小室（0.4微米）中的新生兔胰腺植入物可逆转链脲佐菌素诱导的大鼠糖尿病。血糖、血浆胰岛素和口服葡萄糖耐量试验恢复正常。从糖尿病动物身上取出的经链脲佐菌素处理的离体灌注胰腺不分泌胰岛素，6周后从6只动物体内取出植入物导致所有动物在8天内死于高血糖症。这表明植入物并未导致内源性胰腺功能的重建。6周后取出的三个小室中植入的新生兔胰腺碎块在体外分泌胰高血糖素、胰岛素和胰多肽。未观察到排斥反应。因此，兔新生胰腺植入物可能是治疗需要胰岛素的糖尿病患者的可行疗法。其他非同基因内分泌细胞（即垂体、甲状腺和卵巢）的植入物可能对其他内分泌功能减退综合征有用。
