仓鼠线粒体转运RNA缺乏T和通用的GUUCG序列。
Hamster mitochondrial transfer RNA lacks T and the universal GUUCG sequence.
作者信息
Taylor R H, Varricchio F, Dubin D T
出版信息
Biochim Biophys Acta. 1980 May 30;607(3):521-6. doi: 10.1016/0005-2787(80)90162-8.
PMID:7397181
Abstract
Earlier inferences (based on incorporation of radio-activity from methyl-labeled methionine) that mammalian mitochondrial tRNA lacks ribothymidine ('T') have been confirmed using [14C]uridine as precursor. Moreover, oligonucleotide fingerprint analysis of 32P-labeled samples have shown that hamster mitochondrial tRNA lacks the 'universal' pentanucleotide of conventional tRNA in which T normally occurs, CTpsiCG, in modified or unmodified form.
摘要
早期基于甲基标记甲硫氨酸的放射性掺入所做的推断，即哺乳动物线粒体tRNA缺乏核糖胸腺嘧啶（“T”），已通过使用[14C]尿苷作为前体得到证实。此外，对32P标记样品的寡核苷酸指纹分析表明，仓鼠线粒体tRNA缺乏传统tRNA中通常存在的“通用”五核苷酸CTpsiCG，无论其为修饰形式还是未修饰形式。
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