通过质子磁共振监测核糖胸苷在转运RNA热稳定性中的作用。
Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance.
作者信息
Davanloo P, Sprinzl M, Watanabe K, Albani M, Kersten H
出版信息
Nucleic Acids Res. 1979 Apr;6(4):1571-81. doi: 10.1093/nar/6.4.1571.
Abstract
In order to elucidate the functional role of the modified uridines at position 54 of tRNA, the 270 MHz high-field proton NMR spectra of methionine tRNAs from E. coli, from a mutant thereof, and from T. thermophilus, containing ribothymidine, uridine and 2-thioribothymidine, respectively, have been measured as a function of temperature. A comparison of the NMR melting profiles of the minor nucleosides from these tRNAs shows that the melting temperature of uridine containing tRNA is 6 degrees C lower than that of the wild type tRNA whereas that of the 2-thioribothymidine tRNA is 7 degrees C higher than that of the wild type tRNA. These results, therefore, demonstrate that these modifications serve for stabilization of the tertiary structure of tRNA.
摘要
为了阐明转运核糖核酸（tRNA）第54位修饰尿苷的功能作用，分别测定了来自大肠杆菌及其突变体以及嗜热栖热菌的含核糖胸腺嘧啶核苷、尿苷和2-硫代核糖胸腺嘧啶核苷的甲硫氨酸tRNA在270兆赫高场质子核磁共振（NMR）谱随温度的变化情况。对这些tRNA中稀有核苷的NMR解链曲线进行比较表明，含尿苷的tRNA的解链温度比野生型tRNA低6摄氏度，而含2-硫代核糖胸腺嘧啶核苷的tRNA的解链温度比野生型tRNA高7摄氏度。因此，这些结果表明这些修饰有助于稳定tRNA的三级结构。
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