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1
Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance.通过质子磁共振监测核糖胸苷在转运RNA热稳定性中的作用。
Nucleic Acids Res. 1979 Apr;6(4):1571-81. doi: 10.1093/nar/6.4.1571.
2
Proton magnetic resonance spectra of tRNA-Met-f from Thermus thermophilus.嗜热栖热菌tRNA-Met-f的质子磁共振谱。
Nucleic Acids Res. 1977 Mar;4(3):585-93. doi: 10.1093/nar/4.3.585.
3
Purification and thermal stability of several amino acid-specific tRNAs from an extreme thermophile, Thermus thermophilus HB8.嗜热栖热菌HB8中几种氨基酸特异性转运RNA的纯化及热稳定性
J Biochem. 1980 Jan;87(1):1-13. doi: 10.1093/oxfordjournals.jbchem.a132713.
4
Temperature-dependent biosynthesis of 2-thioribothymidine of Thermus thermophilus tRNA.嗜热栖热菌转运核糖核酸（tRNA）中2-硫代核糖胸苷的温度依赖性生物合成
J Biol Chem. 2006 Jan 27;281(4):2104-13. doi: 10.1074/jbc.M510771200. Epub 2005 Nov 28.
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1H nuclear magnetic resonance of modified bases of valine transfer ribonucleic acid (Escherichia coli). A direct monitor of sequential thermal unfolding.缬氨酸转移核糖核酸（大肠杆菌）修饰碱基的1H核磁共振。序列热解链的直接监测。
Biochemistry. 1975 Aug 12;14(16):3612-8. doi: 10.1021/bi00687a015.
6
CD and NMR studies on the conformational thermostability of 2-thioribothymidine found in the T psi C loop of thermophile tRNA.对嗜热菌转运核糖核酸（tRNA）的TψC环中发现的2-硫代核糖胸苷的构象热稳定性进行的圆二色性（CD）和核磁共振（NMR）研究。
Biochem Biophys Res Commun. 1979 Nov 28;91(2):671-7. doi: 10.1016/0006-291x(79)91574-2.
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Tertiary structure in E. coli tRNA Arg and tRNA Val.大肠杆菌tRNA精氨酸和tRNA缬氨酸的三级结构。
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8
Selective utilization of 2-thioribothymidine- and ribothymidine-containing tRNAs by the protein synthetic systems of Thermus thermophilus HB 8 depending on the environmental temperature.嗜热栖热菌HB 8的蛋白质合成系统根据环境温度对含2-硫代核糖胸苷和核糖胸苷的tRNA进行选择性利用。
J Biochem. 1984 Nov;96(5):1625-32. doi: 10.1093/oxfordjournals.jbchem.a134993.
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Two tRNAIle1 species from an extreme thermophile, Thermus thermophilus HB8: effect of 2-thiolation of ribothymidine on the thermostability of tRNA.来自嗜热栖热菌HB8的两种异亮氨酸tRNA1：核糖胸腺嘧啶2-硫代化对tRNA热稳定性的影响
Biochemistry. 1985 Oct 8;24(21):5711-5. doi: 10.1021/bi00342a004.
10
Environment of ribothymidine in transfer ribonucleic acid studied by means of nuclear Overhauser effect.通过核Overhauser效应研究转移核糖核酸中核糖胸腺嘧啶的环境。
Biochemistry. 1981 Apr 14;20(8):2133-40. doi: 10.1021/bi00511a010.

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RNA modifications stabilize the tertiary structure of tRNAfMet by locally increasing conformational dynamics.RNA 修饰通过局部增加构象动力学来稳定甲硫氨酰 tRNA 的三级结构。
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The hyperthermophilic partners Nanoarchaeum and Ignicoccus stabilize their tRNA T-loops via different but structurally equivalent modifications.高温栖热菌和触火球菌通过不同但结构等效的修饰来稳定它们的 tRNA T 环。
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本文引用的文献

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Rapid ion-exchange chromatographic microanalysis of ultraviolet-absorbing materials and its application to nucleosides.紫外吸收物质的快速离子交换色谱微分析及其在核苷中的应用。
Anal Biochem. 1968 Oct 24;25(1):77-98. doi: 10.1016/0003-2697(68)90083-3.
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Aminoacylation and polypeptide synthesis with tRNA lacking ribothymidine.使用缺乏核糖胸苷的tRNA进行氨酰化和多肽合成。
Biochim Biophys Acta. 1971 May 13;238(2):331-7. doi: 10.1016/0005-2787(71)90100-6.
3
Isolation of mutants of Escherichia coli lac king 5-methyluracil in transfer ribonucleic acid or 1-methylguanine in ribosomal RNA.分离大肠杆菌转移核糖核酸中缺乏5-甲基尿嘧啶或核糖体核糖核酸中缺乏1-甲基鸟嘌呤的突变体。
J Mol Biol. 1970 Jul 14;51(1):83-100. doi: 10.1016/0022-2836(70)90272-x.
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Molecular structure of poly-2-thiouridylic acid, a double helix with non-equivalent polynucleotide chains.聚-2-硫代尿苷酸的分子结构，一种具有不等同多核苷酸链的双螺旋结构。
J Mol Biol. 1974 May 15;85(2):213-9. doi: 10.1016/0022-2836(74)90361-1.
5
Protein synthetic ability of Escherichia coli valine transfer RNA with pseudouridine, ribothymidine, and other uridine-derived residues replaced by 5-fluorouridine.将假尿苷、核糖胸腺嘧啶及其他尿苷衍生残基替换为5-氟尿苷的大肠杆菌缬氨酸转移RNA的蛋白质合成能力。
J Mol Biol. 1974 Sep 15;88(2):313-25. doi: 10.1016/0022-2836(74)90484-7.
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The molecular mechanism of thermal unfolding of Escherichia coli formylmethionine transfer RNA.大肠杆菌甲酰甲硫氨酸转移核糖核酸热解折叠的分子机制。
J Mol Biol. 1974 Jul 25;87(1):63-88. doi: 10.1016/0022-2836(74)90560-9.
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The isolation and sequence analysis of transfer RNA: the use of plaskon chromatography (RPC-5).转运RNA的分离与序列分析：柱层色谱法（RPC - 5）的应用
Biochim Biophys Acta. 1973 Aug 10;319(1):25-36. doi: 10.1016/0005-2787(73)90037-3.
8
Role of modifications in tyrosine transfer RNA. II. Ribothymidylate-deficient tRNA.酪氨酸转运核糖核酸修饰的作用。II. 缺乏核糖胸苷酸的转运核糖核酸
Arch Biochem Biophys. 1973 Jul;157(1):55-62. doi: 10.1016/0003-9861(73)90389-5.
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Effect of ribothymidine in specific eukaryotic tRNAs on their efficiency in in vitro protein synthesis.核糖胸苷在特定真核tRNA中对其体外蛋白质合成效率的影响。
Nature. 1976 May 13;261(5556):159-62. doi: 10.1038/261159a0.
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Physiological and biochemical studies on the function of 5-methyluridine in the transfer ribonucleic acid of Escherichia coli.关于5-甲基尿苷在大肠杆菌转移核糖核酸中功能的生理生化研究。
J Bacteriol. 1975 Oct;124(1):99-111. doi: 10.1128/jb.124.1.99-111.1975.

通过质子磁共振监测核糖胸苷在转运RNA热稳定性中的作用。

Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance.

作者信息

Davanloo P, Sprinzl M, Watanabe K, Albani M, Kersten H

出版信息

Nucleic Acids Res. 1979 Apr;6(4):1571-81. doi: 10.1093/nar/6.4.1571.

DOI:10.1093/nar/6.4.1571
PMID:377228
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327791/
Abstract

In order to elucidate the functional role of the modified uridines at position 54 of tRNA, the 270 MHz high-field proton NMR spectra of methionine tRNAs from E. coli, from a mutant thereof, and from T. thermophilus, containing ribothymidine, uridine and 2-thioribothymidine, respectively, have been measured as a function of temperature. A comparison of the NMR melting profiles of the minor nucleosides from these tRNAs shows that the melting temperature of uridine containing tRNA is 6 degrees C lower than that of the wild type tRNA whereas that of the 2-thioribothymidine tRNA is 7 degrees C higher than that of the wild type tRNA. These results, therefore, demonstrate that these modifications serve for stabilization of the tertiary structure of tRNA.

摘要

为了阐明转运核糖核酸（tRNA）第54位修饰尿苷的功能作用，分别测定了来自大肠杆菌及其突变体以及嗜热栖热菌的含核糖胸腺嘧啶核苷、尿苷和2-硫代核糖胸腺嘧啶核苷的甲硫氨酸tRNA在270兆赫高场质子核磁共振（NMR）谱随温度的变化情况。对这些tRNA中稀有核苷的NMR解链曲线进行比较表明，含尿苷的tRNA的解链温度比野生型tRNA低6摄氏度，而含2-硫代核糖胸腺嘧啶核苷的tRNA的解链温度比野生型tRNA高7摄氏度。因此，这些结果表明这些修饰有助于稳定tRNA的三级结构。