Handley C J, Speight G, Robinson H C, Lowther D A
Biochim Biophys Acta. 1980 Aug 1;631(1):124-9. doi: 10.1016/0304-4165(80)90060-4.
The rate of proteoglycan synthesis by chondrocytes in vitro was depressed by either omitting L-glutamine from the incubation medium or by addition of proteoglycan subunit to the medium. The molecular size distribution on Sepharose 2B of the proteoglycan subunits synthesized by the chondrocytes under these conditions of reduced proteoglycan synthesis was found to be the same as those synthesized by the control cells. Likewise, the molecular size distribution on Sepharose 6B CL of the glycosaminoglycan chains synthesized by the depressed cells was found to be similar to that observed in untreated chondrocytes. This work demonstrates that, under conditions of reduced proteoglycan synthesis, fewer proteoglycan subunits are synthesized by chondrocytes and that the molecular size distribution of these macromolecules is similar to those synthesized by untreated cells.
体外培养的软骨细胞中蛋白聚糖的合成速率，会因在孵育培养基中省略L - 谷氨酰胺或向培养基中添加蛋白聚糖亚基而受到抑制。在蛋白聚糖合成减少的这些条件下，软骨细胞合成的蛋白聚糖亚基在琼脂糖2B上的分子大小分布，与对照细胞合成的蛋白聚糖亚基的分子大小分布相同。同样，合成受抑制的细胞合成的糖胺聚糖链在琼脂糖6B CL上的分子大小分布，与未处理的软骨细胞中观察到的相似。这项工作表明，在蛋白聚糖合成减少的条件下，软骨细胞合成的蛋白聚糖亚基较少，并且这些大分子的分子大小分布与未处理细胞合成的大分子相似。
