Handley C J, Lowther D A
Biochim Biophys Acta. 1979 Jan 18;582(2):234-45. doi: 10.1016/0304-4165(79)90387-8.
Proteoglycans were extracted from the extracellular matrix of cultures of embryonic chick chondrocytes grown at high density and were purified by CsC1 density gradient centrifugation. The chemical, physical and hyaluronate binding properties of the proteoglycans were similar to those observed in proteoglycans from other hyaline cartilages. Proteoglycans in the media were also purified and on analysis showed three populations of proteoglycans to be present. One population had the physical characteristics of a typical proteoglycan subunit and bound hyaluronate, the other two populations were unable to complex with hyaluronate but one had the physical characteristics of the proteoglycan subunit and the other was of smaller molecular weight. The small molecular weight appears to be a product of the enzymatic degradation of the larger molecular weight species.
从高密度培养的胚胎鸡软骨细胞的细胞外基质中提取蛋白聚糖，并通过CsC1密度梯度离心法进行纯化。这些蛋白聚糖的化学、物理和透明质酸结合特性与在其他透明软骨的蛋白聚糖中观察到的特性相似。培养基中的蛋白聚糖也被纯化，分析表明存在三种蛋白聚糖群体。一种群体具有典型蛋白聚糖亚基的物理特征并能结合透明质酸，另外两种群体不能与透明质酸复合，但一种具有蛋白聚糖亚基的物理特征，另一种分子量较小。小分子量似乎是较大分子量物质酶促降解的产物。
