McLeod D L, Shreeve M M, Axelrad A A
Blood. 1980 Aug;56(2):318-22.
When mouse bone marrow cells are seeded in agar cultures containing erythropoietin or pokeweed mitogen stimulated spleen cell conditioned medium plus erythropoietin, megakaryocytes are found mixed with erythroid cells in approximately 40% of the erythropoietic bursts that develop in the cultures. Chromosome spreads of C-metaphases in such "megaerythro bursts" were prepared and stained in situ with a modification of the C-banding technique. In cultures seeded with mixtures of male and female cells, metaphases from individual megaerythro bursts were shown to be either all male of all female but not both. Moreover, tetraploid C-metaphases of megakaryocytes were found to be of the same sex as diploid C-metaphases of erythroid cells in the same megaerythro burst. These results provide evidence that in the mouse, a bipotential progenitor cell exists that has the capacity to give rise to cells of both the megakaryocytic and the erythrocytic lines of differentiation.
当将小鼠骨髓细胞接种于含有促红细胞生成素或商陆有丝分裂原刺激的脾细胞条件培养基加促红细胞生成素的琼脂培养物中时，在培养物中形成的约40%的红细胞生成集落中，可发现巨核细胞与红系细胞混合存在。对这类“巨核红系集落”中的C中期染色体铺片进行制备，并采用改良的C带技术进行原位染色。在用雄性和雌性细胞混合物接种的培养物中，单个巨核红系集落的中期细胞显示要么全为雄性，要么全为雌性，不会二者皆有。此外，发现在同一巨核红系集落中，巨核细胞的四倍体C中期细胞与红系细胞的二倍体C中期细胞性别相同。这些结果证明，在小鼠中存在一种双能祖细胞，它有能力产生巨核细胞系和红细胞系这两种分化细胞。
