利用培养中的分化胚胎细胞检测致畸化合物。
Detection of teratogenic compounds using differentiating embryonic cells in culture.
作者信息
Wilk A L, Greenberg J H, Horigan E A, Pratt R M, Martin G R
出版信息
In Vitro. 1980 Apr;16(4):269-76. doi: 10.1007/BF02618331.
PMID:7399542
Abstract
Methods are described for screening for teratogenic compounds using differentiating neural crest and prechondrogenic limb bud mesenchyme cells in culture. Substances to be tested are either added directly to the culture medium or are combined in a dialysis bag with the postmitochondrial fraction from rat liver and certain cofactors. In the latter case, the compound and its metabolites are gradually released into the medium from the dialysis bag. The results obtained with 14 compounds demonstrate a positive relationship between teratogenicity in vivo and alterations in the growth or the differentiation of the cultured cells.
摘要
本文描述了利用培养中的分化神经嵴和软骨前肢芽间充质细胞筛选致畸化合物的方法。待测试的物质可直接添加到培养基中，或与大鼠肝脏的线粒体后组分及某些辅因子一起置于透析袋中。在后一种情况下，化合物及其代谢产物会从透析袋中逐渐释放到培养基中。用14种化合物得到的结果表明，体内致畸性与培养细胞生长或分化的改变之间存在正相关关系。
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