• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

副溶血性弧菌神奈川现象相关溶血素的简化纯化及生物物理化学特性

Simplified purification and biophysicochemical characteristics of Kanagawa phenomenon-associated hemolysin of Vibrio parahaemolyticus.

作者信息

Miyamoto Y, Obara Y, Nikkawa T, Yamai S, Kato T, Yamada Y, Ohashi M

出版信息

Infect Immun. 1980 May;28(2):567-76. doi: 10.1128/iai.28.2.567-576.1980.

DOI:10.1128/iai.28.2.567-576.1980
PMID:7399675
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC550972/
Abstract

Kanagawa phenomenon-associated hemolysin (K-hemolysin) was purified by Sephadex gel and ion-exchange column chromatography, after the culture supernatant had been adsorbed on and eluted from diethylaminoethyl-Sepharose CL-6B, and acid precipitated. K-hemolysin was a heat-stable and trypsin-susceptible protein with an apparent molecular weight of 44,000, the subunit of which was 22,000. The isoelectric point was 4.9. The minimum hemolytic dose was 0.1 mug/ml. The fifty percent lethal dose by intravenous injection was 1.4 mug. Electron microscopy of the small intestine of suckling mice orally challenged with the highest dose (50 mug) not only showed disappearance of epithelial cell microvilli, but also structural disturbances of the endoplasmic reticulum and mitochondrial swelling. One blueing dose representing permeability factor activity was 0.3 mug, and positive reaction in the rabbit ileal loop appeared at above 125 mug. Besides these data in experimental models, we discovered the appearance of an antibody in patients which neutralizes K-hemolysin during the course of the disease. This finding reinforces our view that K-hemolysin plays a most significant role in the pathogenesis of this enteric human disease.

摘要

将培养上清液吸附于二乙氨基乙基 - 琼脂糖CL - 6B并洗脱，然后进行酸沉淀后，通过葡聚糖凝胶和离子交换柱色谱法纯化神奈川现象相关溶血素（K - 溶血素）。K - 溶血素是一种热稳定且对胰蛋白酶敏感的蛋白质，表观分子量为44,000，其亚基为22,000。等电点为4.9。最小溶血剂量为0.1微克/毫升。静脉注射的半数致死剂量为1.4微克。用最高剂量（50微克）经口攻击的乳鼠小肠的电子显微镜检查不仅显示上皮细胞微绒毛消失，还显示内质网结构紊乱和线粒体肿胀。代表通透因子活性的一个致蓝剂量为0.3微克，在兔回肠袢中，125微克以上出现阳性反应。除了这些实验模型中的数据外，我们还发现患者在疾病过程中出现了中和K - 溶血素的抗体。这一发现强化了我们的观点，即K - 溶血素在这种人类肠道疾病的发病机制中起最重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d85/550972/a5832ddac38b/iai00173-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d85/550972/706f49aa69dc/iai00173-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d85/550972/09fd150979ac/iai00173-0269-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d85/550972/49b16a691f82/iai00173-0269-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d85/550972/d7e2a454fd4c/iai00173-0271-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d85/550972/a5832ddac38b/iai00173-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d85/550972/706f49aa69dc/iai00173-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d85/550972/09fd150979ac/iai00173-0269-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d85/550972/49b16a691f82/iai00173-0269-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d85/550972/d7e2a454fd4c/iai00173-0271-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d85/550972/a5832ddac38b/iai00173-0272-a.jpg

相似文献

1
Simplified purification and biophysicochemical characteristics of Kanagawa phenomenon-associated hemolysin of Vibrio parahaemolyticus.副溶血性弧菌神奈川现象相关溶血素的简化纯化及生物物理化学特性
Infect Immun. 1980 May;28(2):567-76. doi: 10.1128/iai.28.2.567-576.1980.
2
Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin.由神奈川现象阴性副溶血性弧菌临床分离株产生的、与耐热直接溶血素相关的溶血素的纯化及特性分析
Infect Immun. 1988 Apr;56(4):961-5. doi: 10.1128/iai.56.4.961-965.1988.
3
Purification and characterization of thermostable direct hemolysin of Vibrio parahaemolyticus.副溶血性弧菌耐热直接溶血素的纯化与特性分析
Infect Immun. 1973 Nov;8(5):775-80. doi: 10.1128/iai.8.5.775-780.1973.
4
Production, purification, and characterization of botulinolysin, a thiol-activated hemolysin of Clostridium botulinum.肉毒杆菌硫醇激活溶血素肉毒杆菌溶素的生产、纯化及特性分析
Infect Immun. 1992 Jan;60(1):71-8. doi: 10.1128/iai.60.1.71-78.1992.
5
Proceedings: Extraction, purification, and biophysico-chemical characteristics of a "Kanagawa phenomenon"-associated hemolytic factor of Vibrio parahaemolyticus.论文：副溶血性弧菌“神奈川现象”相关溶血因子的提取、纯化及生物物理化学特性
Jpn J Med Sci Biol. 1975 Feb;28(1):87-90.
6
Purification of a TDH-related hemolysin produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus 06: K46.由副溶血性弧菌06:K46的神奈川现象阴性临床分离株产生的一种TDH相关溶血素的纯化
FEMS Microbiol Lett. 1989 Jan 15;48(2):241-5. doi: 10.1111/j.1574-6968.1989.tb03307.x.
7
Comparison of Vibrio parahaemolyticus hemolysin (Vp-TRH) produced by environmental and clinical isolates.环境分离株与临床分离株所产生的副溶血性弧菌溶血素（Vp-TRH）的比较。
FEMS Microbiol Lett. 1992 Apr 15;71(2):157-61. doi: 10.1016/0378-1097(92)90505-i.
8
Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus.与神奈川现象阴性的副溶血性弧菌临床分离株产生的耐热直接溶血素相关的溶血素特性
Can J Microbiol. 1990 Jun;36(6):395-9. doi: 10.1139/m90-069.
9
Characterisation of a haemolysin related to Vp-TDH produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus.对一株神奈川现象阴性的副溶血性弧菌临床分离株产生的与Vp-TDH相关的溶血素的特性研究。
J Med Microbiol. 1995 Feb;42(2):83-90. doi: 10.1099/00222615-42-2-83.
10
Identification of lethal toxin with the thermostable direct hemolysin produced by Vibrio parahaemolyticus, and some physicochemical properties of the purified toxin.副溶血性弧菌产生的致死毒素与耐热直接溶血素的鉴定以及纯化毒素的一些理化性质
Infect Immun. 1976 Jan;13(1):133-9. doi: 10.1128/iai.13.1.133-139.1976.

引用本文的文献

1
Differential Recognition of OmpU by Toll-Like Receptors in Monocytes and Macrophages for the Induction of Proinflammatory Responses.OmpU 通过单核细胞和巨噬细胞中的 Toll 样受体的差异识别诱导促炎反应。
Infect Immun. 2019 Apr 23;87(5). doi: 10.1128/IAI.00809-18. Print 2019 Mar.
2
Phylogenetic and in silico functional analyses of thermostable-direct hemolysin and tdh-related encoding genes in Vibrio parahaemolyticus and other Gram-negative bacteria.副溶血性弧菌及其他革兰氏阴性菌中耐热直接溶血素和tdh相关编码基因的系统发育及计算机功能分析
Biomed Res Int. 2014;2014:576528. doi: 10.1155/2014/576528. Epub 2014 Jul 8.
3

本文引用的文献

1
Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent.用德雷伍德蒽酮试剂定量测定碳水化合物
Science. 1948 Mar 5;107(2775):254-5. doi: 10.1126/science.107.2775.254.
2
The spectrophotometric determination of tyrosine and tryptophan in proteins.蛋白质中酪氨酸和色氨酸的分光光度测定法。
Biochem J. 1946;40(5-6):628-32. doi: 10.1042/bj0400628.
3
Monospecific equine antiserum against cholera exo-enterotoxin.马抗霍乱肠毒素单克隆抗体血清。
Isolation and characterization of a toxic intracellular protein from Vibrio parahaemolyticus.
从副溶血性弧菌中分离和鉴定一种有毒的细胞内蛋白。
World J Microbiol Biotechnol. 1996 Jan;12(1):50-2. doi: 10.1007/BF00327800.
4
Contribution of Vibrio parahaemolyticus virulence factors to cytotoxicity, enterotoxicity, and lethality in mice.副溶血性弧菌毒力因子对小鼠细胞毒性、肠毒性和致死性的贡献。
Infect Immun. 2010 Apr;78(4):1772-80. doi: 10.1128/IAI.01051-09. Epub 2010 Jan 19.
5
Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region.两种调控物通过控制 Vp-PAI 区域基因的表达在副溶血性弧菌肠毒性中发挥重要作用。
PLoS One. 2010 Jan 13;5(1):e8678. doi: 10.1371/journal.pone.0008678.
6
Association of Vibrio parahaemolyticus thermostable direct hemolysin with lipid rafts is essential for cytotoxicity but not hemolytic activity.副溶血性弧菌耐热直接溶血素与脂筏的关联对于细胞毒性是必需的，但对于溶血活性不是必需的。
Infect Immun. 2010 Feb;78(2):603-10. doi: 10.1128/IAI.00946-09. Epub 2009 Nov 23.
7
Hfq regulates the expression of the thermostable direct hemolysin gene in Vibrio parahaemolyticus.Hfq调节副溶血性弧菌中耐热直接溶血素基因的表达。
BMC Microbiol. 2008 Sep 21;8:155. doi: 10.1186/1471-2180-8-155.
8
Purification and characterization of a hemolysin produced by Vibrio mimicus.拟态弧菌产生的一种溶血素的纯化与特性分析
Infect Immun. 1997 May;65(5):1830-5. doi: 10.1128/iai.65.5.1830-1835.1997.
9
Comparison of a fluorogenic assay with a conventional method for rapid detection of Vibrio parahaemolyticus in seafoods.一种用于快速检测海鲜中副溶血性弧菌的荧光测定法与传统方法的比较。
Appl Environ Microbiol. 1996 Sep;62(9):3516-20. doi: 10.1128/aem.62.9.3516-3520.1996.
10
Production of thermostable direct hemolysin by Vibrio parahaemolyticus enhanced by conjugated bile acids.结合胆汁酸增强副溶血性弧菌耐热直接溶血素的产生。
Appl Environ Microbiol. 1996 Aug;62(8):3023-5. doi: 10.1128/aem.62.8.3023-3025.1996.
Infect Immun. 1970 Dec;2(6):691-7. doi: 10.1128/iai.2.6.691-697.1970.
4
Ultraviolet absorption spectra of proteins and amino acids.蛋白质和氨基酸的紫外吸收光谱。
Adv Protein Chem. 1952;7:319-86. doi: 10.1016/s0065-3233(08)60022-4.
5
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
6
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
7
DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.圆盘电泳。一、背景与理论。
Ann N Y Acad Sci. 1964 Dec 28;121:321-49. doi: 10.1111/j.1749-6632.1964.tb14207.x.
8
Enterotoxicity of bacteria-free culture-filtrate of Vibrio cholerae.霍乱弧菌无细胞培养滤液的肠毒性
Nature. 1959 May 30;183(4674):1533-4. doi: 10.1038/1831533a0.
9
Diffusion-in-gel methods for immunological analysis.用于免疫分析的凝胶扩散法。
Prog Allergy. 1958;5:1-78.
10
[A micro-method of immuno-electrophoresis].[免疫电泳的微量方法]
Int Arch Allergy Appl Immunol. 1955;7(2):103-10.